We narrowed to 506 results for: SPY

1. pSPYngK-hp

   Plasmid

   #48118

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   signal peptide of YngK
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpy

   Plasmid

   #29588

   Depositor

   Marc Fiedler

   Insert

   Spy (spy E.coli BL21(DE3))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   processed form of Spy starting at residue 24 (Rem…

   Promoter

   Available since

   April 28, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pTn7xTS_SpyCatcher:mNG

   Plasmid

   #225185

   Purpose

   Used for inserting SpyCatcher fused to mNeonGreen at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SpyCatcher:mNeonGreen
   

   Use

   Tags

   SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTn7xTS_SpyCatcher:AausFP1

   Plasmid

   #225186

   Purpose

   Used for inserting SpyCatcher fused to AausFP1 at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SpyCatcher:AausFP1
   

   Use

   Tags

   SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTn7xTS_SpyCatcher:mCitrine

   Plasmid

   #225187

   Purpose

   Used for inserting SpyCatcher fused to mCitrine at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SpyCatcher:mCitrine
   

   Use

   Tags

   SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTn7xTS_SpyCatcher:mKate2

   Plasmid

   #225188

   Purpose

   Used for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SpyCatcher:mKate2
   

   Use

   Tags

   SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTn7xTS_SpyCatcher:mTFP1

   Plasmid

   #225189

   Purpose

   Used for inserting SpyCatcher fused to mTFP1 at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SpyCatcher:mTFP1
   

   Use

   Tags

   SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAX1_HS_gpN:SpyTag

   Plasmid

   #225193

   Purpose

   Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SpyTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAX1_HS_gpN:SpyTag003

   Plasmid

   #225194

   Purpose

   Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SpyTag003
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpy

    Plasmid

    #220184

    Purpose

    sgRNA expression vector - promoterless backbone

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    promoterless backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    promoterless

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpyA

    Plasmid

    #220185

    Purpose

    sgRNA expression vector - pEM7 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pEM7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpyC

    Plasmid

    #220187

    Purpose

    sgRNA expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpyD

    Plasmid

    #220188

    Purpose

    sgRNA expression vector - pBG28 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBG28

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pspy_STD

    Plasmid

    #165806

    Purpose

    bacterial terminator

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    spy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpyTag003-MBP

    Plasmid

    #133450

    Purpose

    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Use

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SPYtag-GFPnanobody

    Plasmid

    #214837

    Purpose

    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    SPYtag-GFPnanobody
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpyCatcher003-mi3

    Plasmid

    #159995

    Purpose

    Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyCatcher003-mi3
    

    Use

    Tags

    C-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge