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  4. pRSET a MatryoshCaMP6s

    Plasmid
    #100019
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100019-plasmid-map-sequence-id-194295

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  6. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid
    #160881
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160881-plasmid-map-sequence-id-318841

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  7. pCDF-NtXR1AtR2B2NtS1

    Plasmid
    #160879
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: pBAD, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160879-plasmid-map-sequence-id-318813

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  8. pCDF-NtXNtS1R1AtR2B2

    Plasmid
    #160880
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160880-plasmid-map-sequence-id-318839

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  9. pCDF-NtXNtTcR1AtR2NtB2

    Plasmid
    #160889
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-Tc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-Tc: T7, chaperones: T12
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160889-plasmid-map-sequence-id-318862

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  10. pCDF-NtXNtT2R1AtR2NtB2

    Plasmid
    #160886
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T2: T7, chaperones: T9
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160886-plasmid-map-sequence-id-318849

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  11. SPATR-bio

    Plasmid
    #47782
    Purpose
    Expresses enzymatically monobiotinylated full-length SPATR with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised SPATR
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47782-plasmid-map-sequence-id-68814

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  12. pCDF-NtXNtT5R1AtR2NtB2

    Plasmid
    #160888
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T5: T7, chaperones: T11
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160888-plasmid-map-sequence-id-318861

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  13. pCDF-NtXNtT1R1AtR2NtB2

    Plasmid
    #160885
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T1: T7, chaperones: T8
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160885-plasmid-map-sequence-id-318848

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  14. pCDF-NtXNtS5R1AtR2NtB2

    Plasmid
    #160884
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-S5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S5: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160884-plasmid-map-sequence-id-318846

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  15. pCDF-NtXNtS2R1AtR2NtB2

    Plasmid
    #160883
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S2, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S2: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160883-plasmid-map-sequence-id-318843

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  16. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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Showing: 21 - 40 of 3661 results