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A102-GAP-BoCCS

Plasmid

#123287

Purpose

Expression of truncated Glo3 (1-375) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

1-375

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-BoCCS-GRM

Plasmid

#123288

Purpose

Expression of truncated Glo3 (137-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

137-459

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-TurboCas-NLS-Blast

Plasmid

#232346

Purpose

Lentiviral expression vector for dCas9 fused to miniTurboNLS

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA, miniTurbo, V5 and 3xSV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0- Gfap-TagIN-mCherry

Plasmid

#196490

Purpose

To tag endogenous mouse GFAP with mCherry

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry
gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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A102-Par-BoCCS-GRM

Plasmid

#123289

Purpose

Expression of truncated Glo3 (296-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

296-459

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-BOC-COMP5AP-AviTag-9xHis

Plasmid

#157550

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

BOC (BOC Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-BOC-Fc(DAPA)-AviTag-6xHis

Plasmid

#156986

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

BOC (BOC Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCAV1-NLS-mCherry

Plasmid

#187257

Purpose

Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Caveolin-1 (Cav1 Mouse)

Use

Lentiviral

Tags

NLS-mCherry

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shFmnl2-EGFP

Plasmid

#187255

Purpose

Lentiviral expression of shRNA targeting Fmnl2

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Fmnl2 (Fmnl2 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Nsp8-EGFP

Plasmid

#165113

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMega-MaPylRS

Plasmid

#200225

Purpose

The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

Depositor

Alanna Schepartz

Article

Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

Inserts

Pyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA

Use

Tags

none

Expression

Bacterial

Mutation

none

Promoter

proK-lacO and tacI

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Nsp3 -EGFP

Plasmid

#165108

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Nsp7

Plasmid

#165112

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Orf3a-mCherry

Plasmid

#165138

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf3a (ORF3a codon optimized SARS-COV-2)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp12-EGFP

Plasmid

#165116

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp12 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp4-EGFP

Plasmid

#165109

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp14-EGFP

Plasmid

#165119

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge