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Showing: 21 - 40 of 62 results

  4. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60600-plasmid-map-sequence-id-97110

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  7. pZSCAN32-donor

    Plasmid
    #112304
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF434 with GFP
    Depositor
    Kevin White
    Insert
    ZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112304-plasmid-map-sequence-id-228421

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  8. pZSCAN32.1.0-gDNA

    Plasmid
    #112400
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF434
    Depositor
    Kevin White
    Insert
    ZNF434 (ZSCAN32 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112400-plasmid-map-sequence-id-230252

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  15. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

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  19. TFORF0433

    Plasmid
    #142264
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZSCAN32 (ZSCAN32 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    142264-plasmid-map-sequence-id-395755

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  20. TFORF0435

    Plasmid
    #142623
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZSCAN32 (ZSCAN32 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    142623-plasmid-map-sequence-id-397783

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Showing: 21 - 40 of 62 results