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  2. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

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  6. makeTCR_amilCP_control_insert

    Plasmid
    #233942
    Purpose
    control for makeTCR assembly
    Depositor
    Michael Platten
    Insert
    amilCP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23110
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233942-plasmid-map-sequence-id-468272

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  7. pX459_GINS4_gRNA

    Plasmid
    #232734
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232734-plasmid-map-sequence-id-465450

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  8. pUC19_GINS4_dTAG

    Plasmid
    #232735
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232735-plasmid-map-sequence-id-465448

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  12. pINSPECT_SigP:NLuc

    Plasmid
    #197263
    Purpose
    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197263-plasmid-map-sequence-id-389724

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Showing: 21 - 40 of 141943 results