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pMA_Auxiliary 4Δ

Plasmid

#102715

Purpose

Provides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_Ntag_BbsI-Mammalian

Plasmid

#186674

Purpose

N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Mammalian N-terminal Tag

Use

Tags

3xFlag-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID2S

Plasmid

#188648

Purpose

Expresses SpCas9_Target-AID2S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID3S

Plasmid

#188649

Purpose

Expresses SpCas9_Target-AID3S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID3S-UGI

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIAS-1

Plasmid

#184478

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIAS-2

Plasmid

#184479

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLIA CRX (CC#69)

Plasmid

#15128

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pHdCas9-mammalian

Plasmid

#138415

Purpose

Expression of Himar-dCas9 in mammalian cells

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Use

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLIA EnR (CC#29)

Plasmid

#15152

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

Engrailed repressor (en Fly)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

engrailed repressor domain

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

Plasmid

#202661

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBP-PliaG

Plasmid

#112777

Purpose

Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

PliaG promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLIA-E p27 (CC#620)

Plasmid

#15191

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pEF5B-FRT-cilia-APEX

Plasmid

#73186

Purpose

Expresses cilia-APEX in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

cilia-APEX (Nphp3 Glycine max, Mouse)

Use

Tags

internal GFP tag

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDF0317 HvsCas7-11 mammalian expression

Plasmid

#172511

Purpose

Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

HvsCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLIA mNIC myc (CC#341)

Plasmid

#15131

Depositor

Connie Cepko

Article

Bao et al J Neurosci. 1997 Feb 15. 17(4):1425-34.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753.…

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pENTR4 CDS A. thaliana PHOT1

Plasmid

#209187

Purpose

pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT3G45780.1 (PHOT1 Mustard Weed)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLIA CRX EnR (CC#145)

Plasmid

#15155

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Tags

Engrailed Repressor

Expression

Mammalian

Mutation

amino acids 7–108, containing the CRX homeobox

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only