-
-
-
pET28-3'TwStrep
Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
p28BIOHTEV-LIC
Plasmid#167852PurposeBacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purificationDepositorTypeEmpty backboneUseTags6xHis and AviTagExpressionBacterialMutationPromoterAvailable sinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28GSTlinker-LIC
Plasmid#119970PurposeStructure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TBXTA-c006
Plasmid#139755PurposeFor protein expression/crystallography (PDB:6F59)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6ExpressionBacterialMutationG177D; contains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c005
Plasmid#139754PurposeFor protein expression/crystallography (PDB:6F58)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6ExpressionBacterialMutationContains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p28BIOH-LIC
Plasmid#62352PurposeE. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tagDepositorTypeEmpty backboneUseTags6xHis and AviTagExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
ppie.001.301.W257A
Plasmid#137652PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppie (PPIE Human)
UseTagsGST-(His)6-thrombinExpressionBacterialMutation001-301-W257APromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT FL USP7
Plasmid#227214PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationPromoterT7-promoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28GG
Plasmid#104655PurposeLevel 2 cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-GG-PUF
Plasmid#52137PurposeReceiving vector for PUM-HD assembly and expression in E.coliDepositorTypeEmpty backboneUseTagsPUM-HD flanking region 1' and PUM-HD flankin…ExpressionBacterialMutationPromoterT7Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
G392A FL USP7
Plasmid#227217PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationG392APromoterT7-promoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
L469R USP7 catalytic
Plasmid#227212PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL469R; aa 207-560PromoterT7-promoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
N418S FL USP7
Plasmid#227215PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationN418SPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
G392D FL USP7
Plasmid#227216PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationG392DPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
K420E FL USP7
Plasmid#227223PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationK420EPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L299A USP7 catalytic
Plasmid#227213PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL299A; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only
