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Plasmid#89489PurposeshRNA against AMPK alpha1 in mouse/human to knock down protein expressionDepositorUseMouse Targeting and RetroviralTagsExpressionMammalianMutationPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only
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pEGFP-C1-PRKAA2
Plasmid#30310DepositorInsertAMP-activated Protein Kinase α2 (Prkaa1 Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Cherry-N1-PRKAG1
Plasmid#30308DepositorInsertAMP-activated Protein Kinase γ1 (PRKAA1 Human)
UseTagsFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PRKAA2ΔC
Plasmid#30307DepositorInsertAMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)
UseTagsGFPExpressionMammalianMutationdeleted amino acids Ser 527 to Arg 552PromoterAvailable sinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_KD
Plasmid#79011PurposeFor mammalian expression of a kinase dead (K47R) version of AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationK47RPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO AMPKa (11-281)
Plasmid#177851PurposeBacterial Expression of AMPKa1DepositorInsertAMPK a1 (PRKAA1 Human)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBG‐AMPK α1(1‐312)
Plasmid#27632DepositorInsertAMPK α1(1‐312) (Prkaa1 Rat)
UseTagsGSTExpressionMammalianMutationConstitutively active by truncation after am…PromoterAvailable sinceMay 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone1
Plasmid#162119PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-AMPK alpha1 (1-312)
Plasmid#162131PurposeExpression vector for constitutively active AMPK alpha 1DepositorInsertAMPK alpha1 (1-312) (PRKAA1 Rat)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone2
Plasmid#162120PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone3
Plasmid#162121PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgAMPKa1
Plasmid#138704PurposeExpresses a human AMPKa1-targeting sgRNA and Cas9DepositorInsertsgAMPKa1 human (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgAMPKa1
Plasmid#138684PurposeExpresses a human AMPKa1-targeting sgRNA and Cas9DepositorInsertsgAMPKa1 human (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgAMPKa1-Cas9-GFP
Plasmid#208049PurposeLentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1DepositorInsertsgPRKAA1 (PRKAA1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgAmpk1-hU6-sgAmpk2
Plasmid#177224PurposeExpresses Ampk1 and Ampk2 targeting gRNAs and Cre-recombinaseDepositorInsertsgPrkaa1/sgPrkaa2
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2
Plasmid#177233PurposeExpresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinaseDepositorInsertsgNeo1/sgPrkaa1/sgPrkaa2
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only