We narrowed to 3,089 results for: sam

1. nLuc-ΔECD-PTHR1

   Plasmid

   #190011

   Purpose

   Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain

   Depositor

   Sam Gellman

   Article

   Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

   Insert

   nLuc-ΔECD-PTHR1 (PTH1R Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. saposin B^W

   Plasmid

   #161759

   Purpose

   Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

   Depositor

   Sam Gellman

   Article

   Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

   Insert

   saposin B R38_W
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   changed Arginine 38 to Tryptophan

   Promoter

   T7

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NbPT5b-p3

   Plasmid

   #160001

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   Bar (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Agrobacterium tumefaciens Nopaline synthase Promo…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NbBCP1b-p3

   Plasmid

   #160002

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbBCP1b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   Bar (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Agrobacterium tumefaciens Nopaline synthase Promo…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-shSmad2

   Plasmid

   #37051

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

   Insert

   Smad2 (SMAD2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220608

   Purpose

   Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-puro-Smad7-HA

   Plasmid

   #37044

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

   Insert

   Smad7 (SMAD7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. -4200GAMT-P1-pGL4

   Plasmid

   #26804

   Depositor

   Sam Lee

   Article

   Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

   Insert

   Guanidinoacetate N-methyltransferase (GAMT Human)
   

   Use

   Luciferase

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pBabe-puro-TGFβRII-HA

   Plasmid

   #37040

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

   Insert

   TGFβRII
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid

    #220609

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-fDIO-ArgiNLS-AausFP1

    Plasmid

    #220605

    Purpose

    Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

    Plasmid

    #220597

    Purpose

    Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mVenus-Q69M (ME)
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-FLEX-ArgiNLS-EGFP

    Plasmid

    #220601

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0-NbPT5bPro

    Plasmid

    #159416

    Purpose

    Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    NbPT5b Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL0-NbBCP1bPro

    Plasmid

    #159417

    Purpose

    Nicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    NbBCP1b Pro
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL0-BvCYP76AD1

    Plasmid

    #162529

    Purpose

    Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    CYP76AD1
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL0-MjcDOPA5GT

    Plasmid

    #162530

    Purpose

    Mirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    cDOPA5GT
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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