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ULK1 gRNA (BRDN0001147908)

Plasmid

#75738

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-TSF-TEVcs-ULK1

Plasmid

#171416

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO human ULK1 shRNA 8

Plasmid

#27633

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

human ULK1 shRNA 8

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 k46I

Plasmid

#27630

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK1(K46N)

Plasmid

#38197

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 46 to Asparagine

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pcdna6.2- myc ULK1 4SA

Plasmid

#27631

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pQCXIN- myc ULK1 4SA

Plasmid

#27628

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Retroviral

Tags

myc

Expression

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pME18S-3HA-mULK1(K46N)

Plasmid

#22897

Depositor

Masaaki Muramatsu

Article

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 46 to Aspargine

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pQCXIN- myc ULK1 K46I

Plasmid

#27627

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Retroviral

Tags

myc

Expression

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
MBP-TSF-TEVcs-ULK1K46I

Plasmid

#171417

Purpose

For the purifcation of human ULK1 kinase dead complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

kinase dead

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 k46I

Plasmid

#27624

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Expression

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Expression

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-ULK1 (836-1050)-MBP

Plasmid

#203562

Purpose

Mammalian expression of ULK1 (836-1050) with MBP tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ULK1 (ULK1 Human)

Use

Tags

MBP-10His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

Plasmid

#180644

Purpose

Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

ULK1 (ULK1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

Residues 833-1050

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (450-517)-ULK1 (836-1050)-His

Plasmid

#222825

Purpose

Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (363-517)-ULK1 (836-1050)-His

Plasmid

#222824

Purpose

Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST-ATG13 (363-517)-ULK1 (836-1050)

Plasmid

#222822

Purpose

Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF

Plasmid

#203549

Purpose

Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

TwinStrep Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)

Plasmid

#203548

Purpose

Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

TwinStrep Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge