We narrowed to 3,671 results for: Mak

1. pC002 - LshC2C2 locus into pACYC184

   Plasmid

   #79151

   Purpose

   Endogenous LshC2c2 locus cloned into pACYC184

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

   Plasmid

   #79152

   Purpose

   LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Mutation

   *

   Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPbcsx28

   Plasmid

   #89909

   Purpose

   Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   csx28
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBZcsx27

   Plasmid

   #89900

   Purpose

   Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   csx27
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBZCas13b

   Plasmid

   #89898

   Purpose

   Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPbcas13b

   Plasmid

   #89906

   Purpose

   Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBZCas13b-HEPN

   Plasmid

   #89901

   Purpose

   Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R116A/H121A/R1177A/H1182A

   Promoter

   Lac

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZ001 (AacC2c1 locus)

   Plasmid

   #70166

   Purpose

   Expresses AacC2c1 full locus

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   AacC2c1 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZ003 (LshC2c2 locus)

    Plasmid

    #70168

    Purpose

    Expresses LshC2c2 full locus

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    LshC2c2 full locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge