We narrowed to 379 results for: sacas9

1. pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

   Plasmid

   #223158

   Purpose

   Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

   Plasmid

   #223156

   Purpose

   Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 143-224

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

   Plasmid

   #223155

   Purpose

   Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121815

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121820

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

   Plasmid

   #113696

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

   Plasmid

   #109315

   Purpose

   Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   Mttp gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

   Plasmid

   #113695

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Has Service

   AAV6

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

    Plasmid

    #194278

    Purpose

    Expresses gRNA and dSaCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized dSaCas9 KRAB T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hUbC

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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