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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

Plasmid

#164002

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

E1461R

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A

Plasmid

#164001

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

W1460A

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A

Plasmid

#164000

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

M1457A

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A

Plasmid

#163999

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Y1445A

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E

Plasmid

#163998

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

R1427E

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R

Plasmid

#163997

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

E1414R

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R

Plasmid

#163996

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

E1411R

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R

Plasmid

#163994

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

E1397R

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E

Plasmid

#163993

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

R1392E

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E

Plasmid

#163992

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

R1386E

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163059

Purpose

mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

Plasmid

#217321

Purpose

Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

NLRP1

Use

Tags

FLAG and His-TEV

Expression

Insect

Mutation

S969A

Promoter

polyhedrin

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

Plasmid

#166814

Purpose

Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E1322R

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

Plasmid

#166811

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

LL1193EE, P1214R, K1277E

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

Plasmid

#166812

Purpose

Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H1276G

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

Plasmid

#166813

Purpose

Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K1277E

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

Plasmid

#166834

Purpose

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

TEV-GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP Flag Rab32 CA

Plasmid

#44834

Depositor

Matthew Scott

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

Flag

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only