We narrowed to 1,480 results for: asl

1. pWSK129-stm2585

   Plasmid

   #174401

   Purpose

   S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DD-DamWT-LMNB1-IRES2-mCherry

   Plasmid

   #159601

   Purpose

   Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the wild-type allele of Dam.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamWT-LMNB1-IRES2-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DD-DamV133A-IRES2-mCherry

   Plasmid

   #159600

   Purpose

   Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamV133A-IRES2-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dam V133A mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DD-DamWT-IRES2-mCherry

   Plasmid

   #159602

   Purpose

   Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamWT-IRES2-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pShuttle-FEN1DA

   Plasmid

   #35028

   Depositor

   Sheila Stewart

   Article

   Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

   Insert

   FEN1DA (FEN1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D181A--NUCLEASE DEAD POINT MUTANT

   Promoter

   CMV

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pShuttle-FEN1deltaP

   Plasmid

   #35030

   Depositor

   Sheila Stewart

   Article

   Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

   Insert

   FEN1DELTAP (FEN1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   8 AMINO ACID DELETION OF P BOX (amino acids 337–3…

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pFAB808

   Plasmid

   #47853

   Purpose

   BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rrnB T1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLTetO8

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB3045(gRNA XI-3)

   Plasmid

   #73287

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MTK0_003

   Plasmid

   #123925

   Purpose

   Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cas9-sgRNA destination
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MTK0_051

    Plasmid

    #123948

    Purpose

    Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hAAVS1 landing pad destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFAB815

    Plasmid

    #47854

    Purpose

    BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rnpB T1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO9

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDRf1-4CL5-DsRed (JBEI-15461)

    Plasmid

    #87935

    Purpose

    Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTK0_047

    Plasmid

    #123977

    Purpose

    Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hCLYBL CAS9 Destination - HygroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MTK0_057

    Plasmid

    #123950

    Purpose

    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI LP on hAAVS1 (Hygro::mRuby)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p1977 (pUC19-PTDH3-PTEF1)

    Plasmid

    #78230

    Purpose

    Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Bidirectional promoters pTDH3-pTEF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCfB3034(X-3 MarkerFree)

    Plasmid

    #73272

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS423-FjTAL(JBEI-15466)

    Plasmid

    #87936

    Purpose

    Expression in S.cerevisiae of FjTAL

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Insert

    FjTAL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK0_017

    Plasmid

    #123932

    Purpose

    Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI attB tagBFP destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK0_015

    Plasmid

    #123930

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - Insulator + tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MTK3_020

    Plasmid

    #123735

    Purpose

    Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    ERT2-fkbp-spdCa9(205-1368)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB3020(gRNA X-2)

    Plasmid

    #73282

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB3042(gRNA X-4)

    Plasmid

    #73284

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFAB822

    Plasmid

    #47856

    Purpose

    BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    BBa_B1006 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO11

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFAB803

    Plasmid

    #47847

    Purpose

    BIOFAB reporter plasmid for measuring T7 early termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    T7 early terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO2

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-CaMaKIIalpha-mKO2

    Plasmid

    #86156

    Purpose

    Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2016 Oct 11;17(3):720-734. doi

    Insert

    mKO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCfB3050(gRNA XII-5)

    Plasmid

    #73292

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MTK3_001

    Plasmid

    #123952

    Purpose

    Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3XNLS::CRY2PHR::p65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfB3047(gRNA XII-1)

    Plasmid

    #73289

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCfB3046(gRNA XI-5)

    Plasmid

    #73288

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCfB3044(gRNA XI-2)

    Plasmid

    #73286

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCfB3048(gRNA XII-2)

    Plasmid

    #73290

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MTK0_012

    Plasmid

    #123967

    Purpose

    Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hRosa26-BxBI landing pad
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCfB3043(gRNA XI-1)

    Plasmid

    #73285

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB3049(gRNA XII-4)

    Plasmid

    #73291

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JPF0522

    Plasmid

    #124013

    Purpose

    Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. JPF0454

    Plasmid

    #124039

    Purpose

    Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pPGK1-spCAS9-P2A-mAzamiGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. MTK234_027

    Plasmid

    #123907

    Purpose

    Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA1 -hThal5.10-2_hg18
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MTK0_001

    Plasmid

    #123924

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TU-sgRNA - L1/RE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. MTK234_055

    Plasmid

    #123920

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. MTK0_049

    Plasmid

    #123946

    Purpose

    Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hThal5.10-2_hg18 landing pad destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

    Plasmid

    #110747

    Purpose

    Bacterial expression of human USP30 (construct 13i)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    C77A, F348D, M350S, I353E + insertion deletion: 6…

    Promoter

    T7

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pFAB3906

    Plasmid

    #47809

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 14 and BCD2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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48. 6xHis-CPL2/pET28a

    Plasmid

    #172095

    Purpose

    cDNA of CER11/CPL2 cloned into pET28a for protein expression

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    CPL2 (CPL2 Mustard Weed)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. CPL2g/pDONR221

    Plasmid

    #172093

    Purpose

    Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    CPL2 (CPL2 Mustard Weed)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    CPL2

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pEGFP-Flag-stm2585

    Plasmid

    #174397

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge