1,854 results

1. pMALac-OMA-1(1-182)

   Plasmid

   #80350

   Purpose

   Expresses OMA-1 (1-182) in bacteria for protein purification purposes

   Depositor

   Sean Ryder

   Article

   Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

   Insert

   OMA-1 (oma-1 Nematode)
   

   Use

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Encodes AA residues 1-182

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKPY738

   Plasmid

   #72674

   Purpose

   C. elegans let-858 3' UTR (Gateway Entry Clone)

   Depositor

   David Tirrell

   Insert

   let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEG587

   Plasmid

   #40196

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
   

   Use

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   S458A; exon 2 recoded to be RNAi-resistant

   Promoter

   4.4 kb mex-5 promoter fragment

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pJW4.17

   Plasmid

   #62872

   Purpose

   Bacterial expression of MBP-MBK-2 K196R

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-MBK-2 K196R
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   K196R

   Promoter

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGC276

   Plasmid

   #19637

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)

   Use

   Flp/frt, destination vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RGS-6xHis-DRE-1-pcDNA3.1-

   Plasmid

   #52517

   Purpose

   expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Dre-1 (dre-1 Nematode)
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD95.75-cYAP-1

   Plasmid

   #66857

   Purpose

   Generate transgenic C.elegans with cYAP-1 fused to GFP

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

   Insert

   C.elegans YAP-1 (yap-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Serine104 is mutated to alanine

   Promoter

   C. elegans 5'UTR

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pPD95.75-cYAP-1 S104A

   Plasmid

   #66858

   Purpose

   Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

   Insert

   YAP-1 (yap-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Serine104 is mutated to alanine

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. TU#1448_unc-73b_cDNA

   Plasmid

   #82362

   Purpose

   express unc-73b cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   unc-73 b isoform (unc-73 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGC246

    Plasmid

    #19673

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    RNAi; Flp/frt, destination vector

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOX-twk41

    Plasmid

    #16127

    Depositor

    Larry Salkoff

    Article

    Salkoff et al Neuroscience. 2001 . 103(4):853-9.

    Insert

    Tandem pore domain K+ channel (egl-23 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pOX-unc103

    Plasmid

    #16213

    Depositor

    Larry Salkoff

    Insert

    eag-like K+ channels (unc-103 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

13. U653-HTR

    Plasmid

    #24470

    Depositor

    Ron Vale

    Article

    Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

    Insert

    Unc104 (unc-104 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pSR21

    Plasmid

    #69156

    Purpose

    read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCM5.49

    Plasmid

    #21391

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    pal-1 3'utr in pDONRP2RP3
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEG608

    Plasmid

    #40194

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (RNAi resistant) (mex-5 Nematode)
    

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

    Expression

    Worm

    Mutation

    Exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pGC91

    Plasmid

    #19621

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCM3.08

    Plasmid

    #19405

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    pie-1 ORF+3' in pDONR201 (pie-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGC114

    Plasmid

    #19622

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGC231

    Plasmid

    #19644

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGC78

    Plasmid

    #19654

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCM3.09

    Plasmid

    #19406

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    fbf-2 ORF+3' in pDONR201 (fbf-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNAOX-twk5

    Plasmid

    #16105

    Depositor

    Larry Salkoff

    Article

    Wei et al Neuropharmacology. 1996 . 35(7):805-29.

    Insert

    Tandem pore domain K+ channel (twk-5 Nematode)
    

    Use

    Mammalian expression & oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pOX-twk24

    Plasmid

    #16120

    Depositor

    Larry Salkoff

    Article

    Salkoff et al Neuroscience. 2001 . 103(4):853-9.

    Insert

    Tandem pore domain K+ channel (twk-24 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pCM5.35

    Plasmid

    #19395

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    nos-3 3'utr in pDONRP2RP3 (nos-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mg172

    Plasmid

    #34652

    Depositor

    Miriam Goodman

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

    Insert

    slo-1(A1;B1;C0) (slo-1 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Xenopus beta-globulin 3' UTR and Xenopus bet…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGC220

    Plasmid

    #19626

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phlh-12

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBScMXT + egl2

    Plasmid

    #16209

    Depositor

    Larry Salkoff

    Article

    Weinshenker et al J Neurosci. 1999 Nov 15. 19(22):9831-40.

    Insert

    K+-channel KCNQ (egl-2 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pGC163

    Plasmid

    #19635

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)

    Use

    Flp/frt, destination vector

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGC225

    Plasmid

    #19636

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)<4xNLS-tdimer2(12)::let-858(3’)

    Use

    Flp/frt, destination vector

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCM3.05

    Plasmid

    #19601

    Depositor

    Geraldine Seydoux

    Insert

    mex-5 ORF+3' in pDONR201 (mex-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCM5.43

    Plasmid

    #19391

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    daz-1 3'utr in pDONRP2RP3 (daz-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCM5.65

    Plasmid

    #19371

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    mes-3 3'utr in pDONRP2RP3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCCM937

    Plasmid

    #58982

    Purpose

    Expresses avr-15 sgRNA in C.elegans.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    avr-15 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pCCM936

    Plasmid

    #58981

    Purpose

    Expresses avr-14 sgRNA in C.elegans.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    avr-14 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pFM115

    Plasmid

    #40161

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-3C (par-3 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C terminal portion of protein (aa850-1286)

    Promoter

    tac

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pEG459

    Plasmid

    #40088

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S404A) (mex-5 Nematode)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    S404A

    Promoter

    P-lac

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pPK700

    Plasmid

    #38146

    Depositor

    Patricia Kuwabara

    Article

    Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

    Insert

    let-60p::ptc-3::gfp (ptc-3 Nematode)
    

    Use

    Tags

    EGFP

    Expression

    Worm

    Mutation

    Promoter

    let-60

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEG610

    Plasmid

    #40195

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    contains aa 1-355; exon 2 recoded to be RNAi-resi…

    Promoter

    4.4 kb mex-5 promoter fragment

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pGC89

    Plasmid

    #19642

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pNH002

    Plasmid

    #42145

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    N-TAP TAG::[(G4S)3]::mTFP1
    

    Use

    Tags

    TAP TAG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNAOX-twk7

    Plasmid

    #16106

    Depositor

    Larry Salkoff

    Article

    Wei et al Neuropharmacology. 1996 . 35(7):805-29.

    Insert

    Voltage-gated K+ channel (twk-7 Nematode)
    

    Use

    Mammalian expression & oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

44. pcDNAOX-twk13

    Plasmid

    #16110

    Depositor

    Larry Salkoff

    Article

    Wei et al Neuropharmacology. 1996 . 35(7):805-29.

    Insert

    R04F11.4 (twk-13 Nematode)
    

    Use

    Mammalian expression & oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

45. pGC319

    Plasmid

    #19677

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R4-R3)

    Use

    Flp/frt, destination vector

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNH026

    Plasmid

    #42148

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1::linker::TAP TAG
    

    Use

    Tags

    TAP-Tag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNH001

    Plasmid

    #42144

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    TAP-tag
    

    Use

    Tags

    TAP-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCFJ1000

    Plasmid

    #44475

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, PuroR, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGC306

    Plasmid

    #19658

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-1xNLS-DsRed2::let-858(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCM3.35

    Plasmid

    #19404

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    par-5 ORF+3' in pDONR201 (par-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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