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pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163059

Purpose

mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-4mtD3cpv

Plasmid

#36324

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria

Tags

First 29 aa of human COX subunit 8a

Promoter

CMV

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163072

Purpose

Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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LiRTi

Plasmid

#214076

Purpose

Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163057

Purpose

Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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DL146_plenti_TRE_GFP-iPAK4

Plasmid

#187446

Purpose

Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box-Pak4

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-FLuc

Plasmid

#188311

Purpose

Expression of Firefly luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Firefly luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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PU6::unc-119_sgRNA

Plasmid

#46169

Purpose

unc-119 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

unc-119 targeting sgRNA (unc-119 Synthetic)

Use

CRISPR

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDN-Sth1Cas9

Plasmid

#221386

Purpose

Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

Sth1 Cas9

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRF0G-Tyr

Plasmid

#113644

Purpose

Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

MjYRS and tRNA gene

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163077

Purpose

Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Gateway entry vector

Tags

mCherry

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-ER-ZapCY1

Plasmid

#36321

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ER-ZapCY1 high affinity Zn(II) sensor

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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OSCA3.1

Plasmid

#136596

Purpose

Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA3.1 (ERD4 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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