We narrowed to 3,519 results for: Braf;

1. pSP64T-ythdf3-3xflag

   Plasmid

   #164490

   Purpose

   Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

   Depositor

   Antonio Giraldez

   Article

   Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

   Insert

   ythdf3 (ythdf3 Zebrafish)
   

   Use

   Production of mrna for zebrafish injection.

   Tags

   3x-flagtag

   Expression

   Mutation

   Promoter

   SP6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-zfTpo

   Plasmid

   #168198

   Purpose

   Express zebrafish thrombopoietin (Tpo), mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

   Insert

   Thrombopoietin (thpo Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. acta1-mRuby2-dGBP

   Plasmid

   #163855

   Purpose

   Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   dVHH[GFP]
   

   Use

   In vitro transcription of rna

   Tags

   Ruby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. acta1-mRuby2-VHH[GFP]

   Plasmid

   #163856

   Purpose

   Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   VHH[GFP]
   

   Use

   In vitro transcription of rna

   Tags

   Ruby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2pA2-U6:gRNA

   Plasmid

   #63157

   Purpose

   Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. UAS:Zebrabow-B

   Plasmid

   #118216

   Purpose

   For broad gal4 inducible expression of Brainbow in zebrafish

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-lyzC-mito-Dendra2

   Plasmid

   #107590

   Purpose

   Expresses mitochondria localized Dendra2 in zebrafish neutrophils

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   mito-Dendra2
   

   Use

   Tags

   mitochondira localization sequence

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-PLIN2-tdTOMATO

   Plasmid

   #172533

   Purpose

   PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F, and R415H mutations in …

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKHR5

   Plasmid

   #74593

   Purpose

   an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Insert

   FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   alpha-crystallin

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-QFGal4-SV40pA

    Plasmid

    #155126

    Purpose

    In vitro transcription of QFGal4 mRNA

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Expression in zebrafish

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. UAS:Zebrabow-V

    Plasmid

    #118218

    Purpose

    For broad or variegated gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Alex Schier, Albert Pan

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92401

    Purpose

    Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and membrane tag

    Expression

    Mutation

    Promoter

    bactin2

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-MT-mitfa

    Plasmid

    #81231

    Purpose

    expression of zebrafish mitfa

    Depositor

    David Raible, James Lister

    Article

    Lister et al Development. 1999 Sep;126(17):3757-67.

    Insert

    mitfa (mitfa Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    6 myc tags

    Expression

    Mammalian

    Mutation

    contains amino acids 5-412

    Promoter

    sCMV IE94 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-th-sgRNA

    Plasmid

    #65565

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish th locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th sgRNA (th Zebrafish)
    

    Use

    In vitro rna transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. wt1aCreERT2

    Plasmid

    #108464

    Purpose

    Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    wt1aCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. actc1b-mKate2-GPI-pA

    Plasmid

    #109490

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-GPI-pA
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-lyzC-microRNA 722-Dendra2

    Plasmid

    #97130

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

    Insert

    microRNA 722 Dendra2 (mir722 Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVC-Ds-DrU6a:sgRNA-Ds

    Plasmid

    #119069

    Purpose

    To clone sgRNA spacer sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+ dgat1a-FLAG

    Plasmid

    #232143

    Purpose

    Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1a (dgat1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

    Plasmid

    #119070

    Purpose

    Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled1-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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