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pCMV-GABARα1N-HA

Plasmid

#128110

Purpose

Express GABARα1 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra1 (Gabra1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-SNAP-CD4-bla

Plasmid

#179447

Purpose

Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGG-PIP32

Plasmid

#196770

Purpose

Contains RBOHF promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RESPIRATORY BURST OXIDASE HOMOLOG F (RBOH F Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA9

Plasmid

#196108

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-SNAP-CD4-bla

Plasmid

#179452

Purpose

Donor vector to knock in SNAPtag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hMIB2_#9

Plasmid

#75255

Purpose

retroviral shRNA vector against human MIB2, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against MIB2 (human) (MIB2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mClover3-CD4-bla

Plasmid

#179448

Purpose

Donor vector to knock in mClover3 N-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mClover3

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-Luciferase-hvTK-bla

Plasmid

#179450

Purpose

Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

Luciferase

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #1

Plasmid

#179454

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #2

Plasmid

#179455

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #3

Plasmid

#179456

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#1

Plasmid

#189987

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-mClover3-CD4-bla

Plasmid

#179441

Purpose

Donor vector to knock in mClover3 C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mClover3

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hNFATc2/C - AVITEV

Plasmid

#74058

Purpose

retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Promoter

pMSCV-LTRs

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-Halo-CD4-bla

Plasmid

#179445

Purpose

Donor vector to knock in Halotag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hH4-AVITEV

Plasmid

#74051

Purpose

retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Histone-H4 (H4C16 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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