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Showing: 381 - 400 of 8278 results

  1. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

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  2. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Stephen Benkovic
    Insert
    uvsY (uvsY Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228214-plasmid-map-sequence-id-455995

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  3. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

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  4. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

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  5. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

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  6. pEGFP-rUSP19CYT-CAmut

    Plasmid
    #155250
    Purpose
    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155250-plasmid-map-sequence-id-311949

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  7. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

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  8. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid
    #155243
    Purpose
    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155243-plasmid-map-sequence-id-311880

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  9. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid
    #155242
    Purpose
    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155242-plasmid-map-sequence-id-311878

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  10. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid
    #155241
    Purpose
    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155241-plasmid-map-sequence-id-311860

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  11. pRARB.1.0-gDNA

    Plasmid
    #132471
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132471-plasmid-map-sequence-id-267178

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  12. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    T. J. Kappock
    Insert
    delta-8 sphingolipid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73363-plasmid-map-sequence-id-138870

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  13. pJK521

    Plasmid
    #72440
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Unspecified
    Tags
    Expression
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72440-plasmid-map-sequence-id-133778

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  14. pJK323

    Plasmid
    #73495
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73495-plasmid-map-sequence-id-135755

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  15. pJK536

    Plasmid
    #72444
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72444-plasmid-map-sequence-id-136729

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  16. pJK547

    Plasmid
    #72449
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72449-plasmid-map-sequence-id-133874

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  17. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  18. LHP809 - Ub-K63A-CYC1

    Plasmid
    #32166
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K63A
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. LHP327 - Ub-F45A/G76A

    Plasmid
    #32165
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F45A and G76A
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 381 - 400 of 8278 results