We narrowed to 4,627 results for: pcr

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pCRT7 NT Topo arabinose

Plasmid

#73446

Purpose

Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides

Depositor

Jesse Rinehart

Type

Empty backbone

Tags

GST (N-terminal), 6xHis (C-terminal)

Expression

Bacterial

Promoter

pBad

Available Since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Mutation

G12V

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan

Plasmid

#232099

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Hyg

Plasmid

#232098

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBS505 EF1alpha-EGFPcre*

Plasmid

#11955

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCruzHA SIRT1 G261A

Plasmid

#10963

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G261A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCR-Flag-IKKbeta-KM

Plasmid

#15466

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta-KM (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pBS500 EF1alpha-GFPcre

Plasmid

#11920

Depositor

Brian Sauer

Article

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCRISPR Human CASP9 -2

Plasmid

#198420

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -1

Plasmid

#198419

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ERF

Plasmid

#98626

Purpose

Gateway entry TOPO TA cloning vector ERF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ERF (Erf Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCri_L706-antiSH2-OptoMB

Plasmid

#159935

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1825_pCR8-dCasRx-RBM38

Plasmid

#118662

Purpose

Gateway Donor; dCasRx-RBM38 ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR; Gateway donor

Tags

HA Tag

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-HrasG12V

Plasmid

#89887

Purpose

Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-HRasG12V

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3.1 GSK3beta S9A HA

Plasmid

#15994

Depositor

Morris Birnbaum

Article

Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

Insert

GSK3beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A: constitutively active

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-ETV1

Plasmid

#98623

Purpose

Gateway entry TOPO TA cloning vector for ETV1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETV1 (Etv1 Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK4

Plasmid

#98617

Purpose

Gateway entry TOPO TA cloning vector for ELK4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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