We narrowed to 4,504 results for: pcr

1. pCRISPaint-ProteoTuner DD-PuroR

   Plasmid

   #80964

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   ProteoTuner tag
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPaint-NanoLuc-PuroR

   Plasmid

   #80962

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   NanoLuc
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS505 EF1alpha-EGFPcre*

   Plasmid

   #11955

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCR-Flag-IKKalpha-KM

   Plasmid

   #15468

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase alpha-KM (Chuk Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Lysine at 44 to Alanine

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pAC84-pCR8-dCas9

   Plasmid

   #48218

   Purpose

   dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A)
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag

   Expression

   Mutation

   D10A;H840A

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAC1823_pCR8-RBFOX1N-dCasRx-C

   Plasmid

   #118657

   Purpose

   Gateway Donor; RBFOX1N-dCasRx-C ORF; No promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   No promoter

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCruzHA SIRT1 G261A

   Plasmid

   #10963

   Depositor

   Toren Finkel

   Article

   Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G261A

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pBS500 EF1alpha-GFPcre

   Plasmid

   #11920

   Depositor

   Brian Sauer

   Article

   Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

   Insert

   GFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCR8/GW/TOPO-ETV1

   Plasmid

   #98623

   Purpose

   Gateway entry TOPO TA cloning vector for ETV1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ETV1 (Etv1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR-Flag-IKKbeta-KM

    Plasmid

    #15466

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase beta-KM (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Lysine at 44 to Alanine

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCR3.1 GSK3beta S9A HA

    Plasmid

    #15994

    Depositor

    Morris Birnbaum

    Article

    Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

    Insert

    GSK3beta (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A: constitutively active

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pBS594 promoterless EGFPcre

    Plasmid

    #11956

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC1813_pCR8-gSMN2-DN1

    Plasmid

    #118646

    Purpose

    Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCR8/GW/TOPO PCGF1

    Plasmid

    #69770

    Purpose

    Gateway entry vector for PCGF1

    Depositor

    Gerard Cagney

    Insert

    PCGF1 (PCGF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAC1814_pCR8-gSMN2-DN2

    Plasmid

    #118647

    Purpose

    Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC1815_pCR8-gSMN2-DN3

    Plasmid

    #118648

    Purpose

    Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS448 RSV-GFPcre

    Plasmid

    #11919

    Purpose

    Expresses Cre-GFP fusion under the control of the Rous sarcoma virus (RSV) LTR/promoter

    Depositor

    Brian Sauer

    Article

    Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

    Insert

    GFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pCR2.1-promoter-mIL-6

    Plasmid

    #109296

    Purpose

    IL-6 promoter reporter construct

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter in pEGFP-N1 replaced with murine IL-…

    Promoter

    murine IL-6 promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Expression

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge