We narrowed to 10,315 results for: plasmids 101

1. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. B52 (empty plasmid backbone to express 2 sgRNAs)

   Plasmid

   #100708

   Purpose

   Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

   Plasmid

   #100716

   Purpose

   B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting ATP1A1 (cloned using BsmBI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAIP4_3OBS

   Plasmid

   #234227

   Purpose

   Heterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3OBS (TSG101 Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PB-TRE-EGFP-EF1a-rtTA

   Plasmid

   #104454

   Purpose

   PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

   Depositor

   Volker Busskamp

   Article

   Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

   Inserts

   enhanced green fluorescent protein
   reverse tetracycline-controlled transactivator 3
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18

   Plasmid

   #50004

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC13

   Plasmid

   #50003

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC12

   Plasmid

   #50002

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19

   Plasmid

   #50005

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-RARE-luciferase

    Plasmid

    #13458

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Retinoic Acid Receptor Response Element
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHoss1

    Plasmid

    #63158

    Purpose

    Suicide plasmid for efficient gene mutation in Gram-positive bacteria

    Depositor

    Attila Karsi

    Article

    Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

    Insert

    anti-secY-Pxyl/tetO-tetR
    

    Use

    Suicide plasmid for gram-positive bacteria

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PE-SUMO CRYAB

    Plasmid

    #217670

    Purpose

    For expression of SUMO tagged human alpha B crystallin

    Depositor

    Larry David

    Insert

    CRYAB (CRYAB Human)
    

    Use

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFL-SV40-PACT 5'-UTR

    Plasmid

    #115351

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

    Depositor

    Ming-Chih Lai

    Article

    Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

    Insert

    PACT 5'-UTR (PRKRA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. P2RX7-LgBiT(TK) Vector

    Plasmid

    #237101

    Purpose

    Express P2RX7-LgBiT Fusion Protein in Mammalian Cells under a TK promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    P2RX7 (P2RX7 Human)
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    TK

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCAGIG-6xHIS.Mm.RPE65-eGFP

    Plasmid

    #41019

    Depositor

    John Nickerson

    Insert

    RPE65 (Rpe65 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-mNeonGreen

    Plasmid

    #124101

    Purpose

    Expression of the fluorescent protein mNeonGreen in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage was optimized for Homo Sapiens

    Promoter

    CAG

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZNF197.1.0-gDNA

    Plasmid

    #112453

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF197

    Depositor

    Kevin White

    Insert

    ZNF197 (ZNF197 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM5406

    Plasmid

    #132661

    Purpose

    RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM5404

    Plasmid

    #132660

    Purpose

    RSF1010-based broad host-range plasmid with additional RK2 bom site

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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