We narrowed to 2,485 results for: organization Addgene

1. TVBB C-term-mScarlet

   Plasmid

   #169219

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

   Plasmid

   #183411

   Purpose

   piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   Tet-on 3G
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kif5A-GFP-CIBN

   Plasmid

   #102252

   Purpose

   Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   Kif5A-GFP-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP-EGFP-LOV2-Cyb5a

   Plasmid

   #233348

   Purpose

   Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted (Cyb5a 95-134) and N538E

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_erGAP3

   Plasmid

   #78118

   Purpose

   Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

   Depositor

   Javier García-Sancho, Teresa Alonso

   Article

   Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

   Insert

   erGAP3
   

   Use

   Tags

   KDEL and calreticulin signal peptide

   Expression

   Mammalian

   Mutation

   S175G, D180Y, I167V and L15Q in GFP moiety and D…

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.1 mitoAURKA

   Plasmid

   #157750

   Purpose

   Expression of AURKA targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Aurora A without the first 30AA + MTS of CoxVIII

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

   Plasmid

   #201681

   Purpose

   Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

   Depositor

   Emma Rawlins

   Article

   Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

   Inserts

   SFTPC promoter region (2kb upstream)
   TagRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TVBB C-term-mNeongreen

   Plasmid

   #169227

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TVBB N-term-mScarlet

    Plasmid

    #169218

    Purpose

    Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge