Skip to main content
Addgene

We narrowed to 8,240 results for: FIE

Showing: 7961 - 8000 of 8240 results
  1. kiCAP-AAV-MyoAAV3F

    Plasmid
    #224447
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHASW insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. kiCAP-AAV-MyoAAV1C

    Plasmid
    #224438
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDLSTP insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid
    #197264
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Use
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. kiCAP-AAV-MyoAAV2E

    Plasmid
    #224441
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDQGYQ insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. kiCAP-AAV-MyoAAV3D

    Plasmid
    #224445
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYREL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. kiCAP-AAV-MyoAAV3E

    Plasmid
    #224446
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHGVL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. kiCAP-AAV-MyoAAV4B

    Plasmid
    #224449
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYTSV insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. kiCAP-AAV-MyoAAV4D

    Plasmid
    #224451
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHGVL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. kiCAP-AAV-MyoAAV4C

    Plasmid
    #224450
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYTSM insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. kiCAP-AAV-MyoAAV3C

    Plasmid
    #224444
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYSSV insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. kiCAP-AAV-MyoAAV3B

    Plasmid
    #224443
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYSGL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid
    #197265
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. pFUSEss-CHIg-mG1_CH3-3

    Plasmid
    #105853
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.
    Depositor
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH3 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pJW1595

    Plasmid
    #121062
    Purpose
    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Insert
    mKate2^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. pFUSEss-CHIg-mG1_CH2-3

    Plasmid
    #105851
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH2.
    Depositor
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH2 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pFUSEss-CHIg-mG1_CH1h-3

    Plasmid
    #105850
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH1 and hinge.
    Depositor
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH1 and hin…
    Promoter
    hEF1-HTLV prom
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pFUSEss-CHIg-mG1_h-3

    Plasmid
    #105854
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived hinge.
    Depositor
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with hinge deriv…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  22. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 7961 - 8000 of 8240 results