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pcDNA3-ABKAR-Kras

Plasmid

#84637

Purpose

Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras

Tags

6xHis, C-terminal targeting sequence from Kras, C…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Arp3_pLib

Plasmid

#173677

Purpose

Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp3 (ACTR3 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1(H40,L61)

Plasmid

#11403

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine. Th…

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2A1

Plasmid

#67076

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2A1 (GTF2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF-PY3 mutant

Plasmid

#17799

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pBabeHygro-GATA2-Ala(381-385)

Plasmid

#1416

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

alanine substitutions at amino acids 381-385

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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p177 Dync1i2.C (Ex 1b)

Plasmid

#26445

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p196 Dync1i2.A (Ex 1a)

Plasmid

#26446

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p169 Dync1i1.C

Plasmid

#26440

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform C (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
p173 Dync1i2.A (Ex1b)

Plasmid

#26443

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p176 Dync1i2.B (Ex1b)

Plasmid

#26444

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform B (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p197 Dync1i2.B (Ex1a)

Plasmid

#26447

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform B (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pUAS-Tum

Plasmid

#37304

Depositor

Norbert Perrimon

Article

Harrison et al EMBO J. 1995 Jun 15;14(12):2857-65.

Insert

hopTum-1 (hop Fly)

Expression

Insect

Mutation

Tul-l mutation is a G to A transversion at nucleo…

Promoter

Hsp70

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB7

Plasmid

#19557

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pet 3d beta tentacle delete CP

Plasmid

#13300

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)

Expression

Bacterial

Mutation

CP alpha subunit is intact with a mutated beta C-…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
p172 Dync1i2.E (Ex 1b)

Plasmid

#26442

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform E (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p171 Dync1i1.F

Plasmid

#26441

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform F (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
2. p167 Dync1i1.D

Plasmid

#26439

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF-PY1 mutant

Plasmid

#17798

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf164

Plasmid

#12852

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf164

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
p47 PX(1-121)/p40phox(136-339)

Plasmid

#19018

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox/p47phox (NCF4, NCF1 Human)

Tags

His

Expression

Bacterial

Mutation

insert containing amino acids 1-121 of p47phox fu…

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-PCNA

Plasmid

#199702

Purpose

Visualization of cell cycle progression and subdivision of S phase living mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Mol Biol 2016, 5:2. doi: 10.4172/2168-95

Insert

PCNA (PCNA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)

Plasmid

#240254

Purpose

Non-attenuated SFV vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_rTetR-VP16 (3'UTR)_RLEloop

Plasmid

#240257

Purpose

Attenuated PROTEUS vector with rtTA-3G transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

rTetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_TetR-VP16 (3'UTR)_RLEloop

Plasmid

#240259

Purpose

Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

TetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac_eIF2_gamma

Plasmid

#248693

Purpose

Express the human eIF2 gamma subunit in insect cells or clone it in other vectors

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF2 gamma (EIF2S3 Human)

Tags

3C STREP 2

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV2-Sst44-mTagBFP2

Plasmid

#211807

Purpose

Expresses BFP in a subset of cortical somatostatin neurons.

Depositor

Christopher Harvey

Article

Green et al Nature. 2023 Aug;620(7973):366-373. doi:

Insert

mTagBFP2

Use

AAV

Tags

NLS, FLAG

Expression

Mammalian

Promoter

Sst44 enhancer, modified CMV promoter

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac_eIF2_beta

Plasmid

#248692

Purpose

Express the human eIF2 beta subunit in insect cells or clone it in other vectors

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF2 beta (EIF2S2 Human)

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac_eIF2_alpha

Plasmid

#248691

Purpose

Express the human eIF2 alpha subunit in insect cells or clone it in other vectors

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

human eIF2 alpha (EIF2S1 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pFB-CMV-DIO-MTS-roGFP-WPRE

Plasmid

#245375

Purpose

Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

Depositor

D. James Surmeier

Article

Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

Insert

roGFP

Use

AAV

Tags

mitochondrial-targeting sequence of the S. pombe …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DEST (JDW 471)

Plasmid

#242573

Purpose

Gateway destination vector with a CAGGS promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway subcloning

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β6β1-EGFP

Plasmid

#242829

Purpose

Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)

Expression

Mammalian

Mutation

Integrin β6 chimera containing the cytoplasmic ta…

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-SV40

Plasmid

#226453

Purpose

For subcloning of SV40 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

SV40

Use

Cloning vector

Mutation

No

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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NFlhb-t2a-GFP

Plasmid

#194357

Purpose

over expression vector for killifish lhb tagged with GFP

Depositor

Itamar Harel

Article

Moses et al Nat Aging. 2024 Jun;4(6):791-813. doi: 1

Insert

luteinizing hormone subunit beta

Use

Lentiviral

Tags

t2a-GFP

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#224763

Purpose

In vitro transcription for Oocyte expression of hTRAAK(Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

Q258K

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1

Plasmid

#224743

Purpose

In vitro transcription for Oocyte expression of mTREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE:hTRAAK

Plasmid

#224761

Purpose

In vitro transcription for Oocyte expression of hTRAAK

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE:hTRAAK(S118C)

Plasmid

#224762

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

S118C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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