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pESC-URA-Ub-G76A-Rnq1-GFP

Plasmid

#21052

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP and ubiquitin

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pBBRBB-Ppuf843-1200-PR

Plasmid

#50963

Purpose

Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Article

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

Proteorhodopsin

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

Ppuf843-1200

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_WT

Plasmid

#62646

Purpose

Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
LV-H2B-mRFP1

Plasmid

#47376

Purpose

This was used as a nuclear marker

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

H2B

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG FLAG B56 gamma3

Plasmid

#13803

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma 3 Flag (PPP2R5C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-dSa-VP64

Plasmid

#99680

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64

Mutation

dead Cas9

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

C1(1-29)-APEX2-GFP

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST26-hOPTN-D474N

Plasmid

#23051

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

Optineurin (OPTN Human)

Tags

His

Expression

Mammalian

Mutation

D474N

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only
LHP1463 - INP52

Plasmid

#32432

Depositor

Linda Hicke

Article

Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-2

Insert

INP52 (INP52 Budding Yeast)

Expression

Yeast

Promoter

Lac

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-mir-92

Plasmid

#64092

Purpose

expresses miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

miR-92 (MIR92A1 Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pExp_TRE:EBFP2

Plasmid

#51793

Purpose

Inducible expression of EBFP2 with rtTA for input fluorescence

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

EBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 TAP

Plasmid

#17528

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5B ActRIB-HA T206D

Plasmid

#27223

Depositor

Joan Massague

Article

Attisano et al Mol Cell Biol. 1996 Mar . 16(3):1066-73.

Insert

ActRIB (ACVR1B Human)

Tags

HA

Expression

Mammalian

Mutation

T206D

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pME-TagRFPt-NS

Plasmid

#75343

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagRFPt

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Tags

3xFlag and myc

Expression

Bacterial

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-221-P3-P5

Plasmid

#186350

Purpose

Gateway donor vector with AttP3 AttP5 recombination sites

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway donor vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUCdeltaSS-ERE

Plasmid

#108217

Purpose

Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

Depositor

Didier Picard, Keith Yamamoto

Article

Picard et al Nature 1990 Nov 8;348(6297):166-8.

Insert

ERE-CYC1-lacZ

Expression

Yeast

Promoter

CYC1

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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