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pMIG-hNFATc2/C - AVITEV

Plasmid

#74058

Purpose

retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-Elf5 gRNA

Plasmid

#128836

Purpose

gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

mElf5 gRNA (Elf5 Mouse)

Use

CRISPR

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pmx-puro-MiGT

Plasmid

#172399

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i, Gata4, Tbx5 (Gata4, Tbx5, Mef2c Mouse)

Use

Retroviral

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-747_CD19-28z_CAR_TFAP4

Plasmid

#207489

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-BRCA1 3'UTR

Plasmid

#29477

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

BRCA1 3'UTR (BRCA1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO5-SgHottip-GFP-CRISPRa

Plasmid

#134990

Purpose

dCas9-mediated activation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAFNF-PSDd1.2-GFP

Plasmid

#125581

Purpose

Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

PSDd1.2-GFP (Dlg4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-LB2O

Plasmid

#188036

Purpose

Fusion of human MYC Transactivation Domain (TAD) MYC Box II (MBII) with human OCT4 protein expressed in pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

MYC TAD MBII region fused with OCT4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a C2AB

Plasmid

#40051

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a C2AB (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2AB domains only (aa 597-910)

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA4 3xFLAG Halo delta 122-130 nsp1

Plasmid

#175425

Purpose

Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 122-130 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha5 pcDNA1 neo

Plasmid

#13588

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 5 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-AURKB 3'UTR

Plasmid

#29475

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

AURKB 3"UTR (AURKB Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Slp2-a E11A/R32A

Plasmid

#40055

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a E11A/R32A (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Glutamate 11 to Alanine, Arginine 32 to Alanine

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
integrin alpha9 / alpha4 pcDNA1 neo

Plasmid

#13587

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 4 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha2 pcDNA1 neo

Plasmid

#13586

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 2 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pET14-H2Kb-ST3

Plasmid

#223221

Purpose

Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)

Tags

Spytag

Expression

Bacterial

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-TAF5-FL

Plasmid

#209051

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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