159,557 results

1. pmScarlet-i_alphaTubulin_C1

   Plasmid

   #85047

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Alpha-Tubulin (TUBA1B Human)
   

   Use

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

   Plasmid

   #32477

   Purpose

   Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAML141F,Y115F-5HT3HC
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Jan. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK2_023

   Plasmid

   #123715

   Purpose

   Encodes pCMV as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pCMV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBC-SV40NLS-Pfv-Sapphire-PURO

   Plasmid

   #203654

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CMV-evoCjCas9-BlastR

   Plasmid

   #194059

   Purpose

   Mammalian expression of evoCjCas9 nuclease

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CamKIIa-ChR2-EGFP

   Plasmid

   #114216

   Purpose

   Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Channelrhodopsin 2
   

   Use

   AAV and Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa 1.3 kb

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FPR1-Tango

   Plasmid

   #66283

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR1 (FPR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pegRNA_GBA-4

   Plasmid

   #199274

   Purpose

   pegRNA used to correct GBA (c. 1226 A > G) mutation

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   GBA 1226GtoA pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-PM-TORCAR

   Plasmid

   #64930

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEVOL_PylRS(WT)-tRNA

    Plasmid

    #223512

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD, rrnB, and glnS and proK

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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