164,304 results

1. ALPK1 gRNA (BRDN0001146875)

   Plasmid

   #76084

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FCGR2a-bio-His

   Plasmid

   #51754

   Purpose

   Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FCGR2a (FCGR2A Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T-1-6AlanineHE1

   Plasmid

   #165098

   Purpose

   bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1

   Depositor

   Arthur Haas

   Insert

   Uba1 (UBA1 Human)
   

   Use

   Tags

   GST-6-alanine

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

   Plasmid

   #205152

   Purpose

   Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   FRT-GFP-FRT-uORF-dCBEevoCDA1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-miR-34b/34c

   Plasmid

   #63933

   Purpose

   MSCV vector for overexpressing mouse miR-34b/34c

   Depositor

   Lin He

   Article

   Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

   Insert

   microRNA 34b/34c (Mir34c, Mir34b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pICH54011

   Plasmid

   #48065

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. EGFP-GRAM-W

   Plasmid

   #211701

   Purpose

   Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXB3-Flag PLK1 T210D

    Plasmid

    #68272

    Purpose

    bacterial expression of Plk1 T210D mutant

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    T210D

    Promoter

    T7

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only