We narrowed to 37,833 results for: KAN

1. WARS_pNIC-CTB10H

   Plasmid

   #153058

   Purpose

   Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   WARS (WARS1 Human)
   

   Use

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBacMam5_N_Flag_His8_eGFP_3C

   Plasmid

   #178816

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, His8, eGFP, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTKIP-neo

   Plasmid

   #41064

   Depositor

   Edward Cox, Thomas Kuhlman

   Article

   Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)

   Plasmid

   #228044

   Purpose

   Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.

   Depositor

   Wade Harper

   Insert

   CASP1[CARD]-mSG2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ecALDH

   Plasmid

   #67921

   Purpose

   Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Insert

   Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTRDsRedEx2

   Plasmid

   #22451

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   DsRedExpress
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBacMam6_C_3C_His8_Stop

   Plasmid

   #178810

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   3C, His8, Stop codon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEos3.2-Nup50-N-10

   Plasmid

   #57479

   Purpose

   Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   Nup50 (NUP50 Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RAB25

    Plasmid

    #26163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB25:HPC038-B06:C25773 (RAB25 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. mCherry-Myotilin-C-14

    Plasmid

    #55109

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Myotilin (MYOT Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mEmerald-PTK2-C-10

    Plasmid

    #54240

    Purpose

    Localization: Focal Adhesion Kinase, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid

    #219989

    Purpose

    betaglobin-5'UTR-NanoLuc mRNA synthesis

    Depositor

    Simon Alberti

    Insert

    betaglobin-5'UTR-NanoLuc (HBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid

    #64921

    Purpose

    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPE1 (PCBP1 Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLNHA-C1-HseIF4E_K

    Plasmid

    #146732

    Purpose

    Mammalian Expression of HseIF4E

    Depositor

    Elisa Izaurralde

    Insert

    HseIF4E (EIF4E Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mPlum-LC-Myosin-N-7

    Plasmid

    #55983

    Purpose

    Localization: Light Chain Myosin, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    LC-Myosin (Myl9 Mouse)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pJAK1.A

    Plasmid

    #29460

    Depositor

    Zakaria Hmama

    Article

    Sun et al Plasmid. 2009 Nov . 62(3):158-65.

    Type

    Empty backbone

    Use

    Mycobacteria expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Hsp60

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTC-mcs

    Plasmid

    #20317

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

    Insert

    multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEmerald-Tropomyosin-N-14

    Plasmid

    #54290

    Purpose

    Localization: Actin, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Tropomyosin (TPM1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. mCerulean-TFR-20

    Plasmid

    #55391

    Purpose

    Localization: Transferin Receptor, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Insert

    TFR (TFRC Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

21. RAB27B

    Plasmid

    #25545

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB27B (RAB27B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pJM104: pTrc RFP on pCM66T

    Plasmid

    #74745

    Purpose

    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sfCherry11-620W-PTPN22

    Plasmid

    #195386

    Purpose

    Mammalian expression of a human PTPN22 mutant (620W) with sfCherry(11) tag

    Depositor

    Patrick Concannon

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    sfCherry(11)

    Expression

    Mammalian

    Mutation

    R620W (rs2476601)

    Promoter

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RAB31

    Plasmid

    #25552

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB31 (RAB31 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

25. RAB26

    Plasmid

    #25528

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB26 (RAB26 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

26. R777-E151 Hs.PAK3

    Plasmid

    #70435

    Purpose

    Gateway ORF clone of human PAK3 [NM_002578.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PAK3 (PAK3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RAB30

    Plasmid

    #25542

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB30 (RAB30 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mRuby-Rab4a-7

    Plasmid

    #55878

    Purpose

    Localization: Ras GTPase, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. mApple-SiT-N-15

    Plasmid

    #54948

    Purpose

    Localization: Golgi TGN, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

31. Sperm whale Mb 64Y68F in pVP80K

    Plasmid

    #86956

    Purpose

    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

    Depositor

    John Olson

    Insert

    Mb 64Y68F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    64Y68F

    Promoter

    T5

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PA-mCherry1-PDHA1-N-10

    Plasmid

    #57193

    Purpose

    Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMGAHisTev5B

    Plasmid

    #37221

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF5B
    

    Use

    Tags

    His

    Expression

    Mutation

    N-term truncation (contains aa 396-1002)

    Promoter

    Available since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pMN234

    Plasmid

    #32108

    Depositor

    Michael Niederweis

    Article

    Stephan et al Gene. 2004 Dec 8;343(1):181-90.

    Insert

    flpe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    imyc

    Available since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCM5.47

    Plasmid

    #17254

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Sep 23. ():.

    Insert

    pie-1 3'utr in pDONRP2RP3
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pETM-41-HsNot6_N

    Plasmid

    #147011

    Purpose

    Bacterial Expression of HsNot6

    Depositor

    Elisa Izaurralde

    Insert

    HsNot6 (CNOT6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. YARS_pNIC-CTB10H

    Plasmid

    #153059

    Purpose

    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    YARS (YARS1 Human)
    

    Use

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RAB9B

    Plasmid

    #25625

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB9B (RAB9B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

39. pSheLL

    Plasmid

    #37319

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2004 Jan;78(2):751-7.

    Insert

    BPVL1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. RAB2B

    Plasmid

    #25534

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB2B (RAB2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

41. mTagRFP-T-Rab4a-7

    Plasmid

    #58025

    Purpose

    Localization: Ras GTPase, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

42. Preprotachykinin A

    Plasmid

    #130664

    Purpose

    Expresses human Preprotachykinin A in mammalian cells

    Depositor

    Michael Kressel

    Insert

    Preprotachykinin A (TAC1 Human)
    

    Use

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMS051

    Plasmid

    #137025

    Purpose

    SapTrap mScarlet-I-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized mScarlet
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mScarlet

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mRuby-PCNA-19-NLS-4

    Plasmid

    #55876

    Purpose

    Localization: Nucleus, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Use

    Tags

    3xNLS and mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

45. RBKS

    Plasmid

    #25544

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RBKS (RBKS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

46. HT7-mTurquoise2-KDEL

    Plasmid

    #188571

    Purpose

    Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    HaloTag7
    

    Use

    Tags

    KDEL and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pT7-EGFP-C1-HsYTHDF3_AB

    Plasmid

    #148313

    Purpose

    Mammalian Expression of HsYTHDF3

    Depositor

    Elisa Izaurralde

    Insert

    HsYTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. mRuby-Vinculin-23

    Plasmid

    #55885

    Purpose

    Localization: Focal Adhesions, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

49. pRARB-donor

    Plasmid

    #132517

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RARB (RARB Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Nup54-mEGFP494(0)

    Plasmid

    #163426

    Purpose

    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of the mEGFP

    Depositor

    Sanford Simon

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge