We narrowed to 165,214 results for: addgene

1. pMB2-D-TelR

   Plasmid

   #204990

   Purpose

   Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mc2 blast hPGK

   Plasmid

   #206229

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mc2 hygro hPGK

   Plasmid

   #206228

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mc2 puro hPGK

   Plasmid

   #206227

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mc2 neo hPGK

   Plasmid

   #206226

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mc2 hygro EF1a

   Plasmid

   #206222

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mc2 hygro

   Plasmid

   #206216

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIT318

   Plasmid

   #204508

   Purpose

   rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematode

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   rpl-21p::2xHA::C04F12.1 (C. elegans)
   

   Tags

   2xHA

   Expression

   Worm

   Promoter

   rpl-21 (C. elegans)

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIT246

   Plasmid

   #204507

   Purpose

   csr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematode

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   2xFLAG::csr-1 (C. elegans) (csr-1 Nematode)
   

   Tags

   2xFLAG

   Expression

   Worm

   Promoter

   csr-1 (C. elegans)

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TT 3xFLAG-DYNLL2

    Plasmid

    #192296

    Purpose

    Gateway entry vector for an inducible 3xFLAG-DYNLL2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    DYNLL2 (DYNLL2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tornado-RNA sensor for survivin

    Plasmid

    #185405

    Purpose

    circular RNA-scaffolded sensing modules for survivin

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    circular RNA-scaffolded sensing modules
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    U6

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUiLMO2W

    Plasmid

    #129480

    Purpose

    3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human ubiquitin C

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-Rtn2b-mNeon-TurboID

    Plasmid

    #186612

    Purpose

    Third generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 2 isoform B (RTN2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

    Plasmid

    #193980

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2_SulR(CDS)

    Plasmid

    #186423

    Purpose

    provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Cds (part b3-b4-b5), goldenbra…

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D908A

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-Bik-del72-136-pEGFP-C3

    Plasmid

    #191411

    Purpose

    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).

    Depositor

    David Andrews

    Insert

    Bcl-2-interacting killer (BIK Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    with deletion of amino acids 72-136 (del72-136) i…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK411.RR1

    Plasmid

    #192644

    Purpose

    Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    BBa_J23119-RR1
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCL.103

    Plasmid

    #184988

    Purpose

    Express Cas9-P2A-Eco1RT

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-P2A-Eco1RT
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL.027

    Plasmid

    #184964

    Purpose

    Express -Eco1 RT and ncRNA in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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