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We narrowed to 10,563 results for: CHL

Showing: 10051 - 10100 of 10563 results
  1. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    Promoter
    P-LlacO-1
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pPgiCas13b-gRNA-1

    Plasmid
    #184834
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Depositor
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pRB116.2

    Plasmid
    #181946
    Purpose
    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Insert
    TorR-mNG; mCherry; tetR (AX04_RS23485, torR Synthetic, torR - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pRB120.2

    Plasmid
    #181947
    Purpose
    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Insert
    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 Synthetic, torR - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pSBK.136

    Plasmid
    #187220
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively
    Depositor
    Insert
    Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    A: pSalTTC; B: pTet*
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid
    #186413
    Purpose
    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
    Depositor
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pEPQD1CB0768

    Plasmid
    #177058
    Purpose
    MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Insert
    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pEPQD1CB0124

    Plasmid
    #177056
    Purpose
    MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Insert
    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pEPQD1CB0120

    Plasmid
    #177052
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Insert
    major latex protein-like (NmMLPL) from Nepeta mussinii
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pEPQD0CM0065

    Plasmid
    #177019
    Purpose
    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT
    Depositor
    Insert
    1-deoxy-D-xylulose 5-phosphate synthase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    transit peptide removed, amplified from cDNA, con…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pTF101.1gw3

    Plasmid
    #172181
    Purpose
    A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.
    Depositor
    Insert
    attR3-CmR-ccdB-attR4
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    lacUV5 promoter
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. IBMc367

    Plasmid
    #161947
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20
    Depositor
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. IBMc228

    Plasmid
    #161946
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR
    Depositor
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

    Plasmid
    #159838
    Purpose
    Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays
    Insert
    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. RA35

    Plasmid
    #141126
    Purpose
    Expresses dimeric LacI repressor (LacI-11) under Rhl promoter
    Depositor
    Inserts
    dimeric LacI (LacI-11) (lacI Synthetic)
    AiiA (AQ980_RS12585 )
    Use
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    truncated/dimeric version of LacI (missing last 1…
    Promoter
    engineered rhl promoter (responds to C4-HSL) and …
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pSB1C3_mTq-Tat (c-plasmid)

    Plasmid
    #127635
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.
    Depositor
    Insert
    mTurquoise2
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  21. pKD277

    Plasmid
    #125098
    Purpose
    Expression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    SO_2193(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 10051 - 10100 of 10563 results