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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100845

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJ609-dCas9-KR-puro

Plasmid

#158478

Purpose

Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

Depositor

Brendan Price

Article

House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

Insert

dCas9-KR

Use

CRISPR

Tags

KillerRed

Expression

Mammalian

Mutation

Cas9m4 (D10A+D839A+H840A+N863A)

Promoter

CMV

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-WT

Plasmid

#30370

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

wild type

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pGWB602-OEP7-mVenus

Plasmid

#213864

Purpose

Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-Y138L

Plasmid

#30378

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y138L (PTEN Human)

Expression

Mammalian

Mutation

Y138L

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
AAV2-hSyn-G-Flamp1-mut

Plasmid

#188570

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

AAV

Tags

His tag

Promoter

hSyn

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_H2A_C1

Plasmid

#85053

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Tags

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-G129E

Plasmid

#30377

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-G129E (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Promoter

hSynapsin1

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB

Plasmid

#137001

Purpose

mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pZyxin-miRFP703

Plasmid

#79999

Purpose

Zyxin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Zyxin (ZYX Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a-EGFP

Plasmid

#205307

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0

Plasmid

#164606

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a-EGFP

Plasmid

#205309

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.2

Plasmid

#111054

Purpose

Expresses dLight1.2 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMA3287

Plasmid

#46883

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase, Y147A mutant (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Y147A

Promoter

TRE-Tight

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

Plasmid

#202608

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

Plasmid

#132410

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dEsCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R295A; H300A; R849A; H854A

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RdLight1

Plasmid

#125707

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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