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We narrowed to 26,940 results for: sta

Showing: 4021 - 4040 of 26940 results

  3. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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  4. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

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  5. pHochTypeIII

    Plasmid
    #214039
    Purpose
    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Cas10
    Use
    CRISPR
    Tags
    Expression
    Mutation
    WT
    Promoter
    T7 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214039-plasmid-map-sequence-id-424943

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  7. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  11. pICE-FLAG-NAT10-siR-WT

    Plasmid
    #59365
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    human NAT10 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Mutations to render cDNA resistant to a siRNA
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59365-plasmid-map-sequence-id-92043

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  12. pIgK-2.1-FLAG

    Plasmid
    #225829
    Purpose
    Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG-tagged monoclonal antibodies for use as tag-specific ELISA standards.
    Depositor
    Gabriel Victora
    Article
    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:
    Insert
    Mouse anti-CGG IgK chain, Flag-tagged
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    225829-plasmid-map-sequence-id-448813

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  15. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Use
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Promoter
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  17. pICE-FLAG-NAT10-siR-G641E

    Plasmid
    #59366
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    NAT10 NM_024662 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    G641E and silent mutations to render the cDNA res…
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59366-plasmid-map-sequence-id-92067

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Showing: 4021 - 4040 of 26940 results