Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 10,805 results for: CHL

Showing: 10101 - 10150 of 10805 results

  6. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

    Enlarge

  8. YFVdelNS1-Cre

    Plasmid
    #179952
    Purpose
    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    YFV-17D lacking nonstructural protein 1 (NS1), but with Cre
    Use
    Transneuronal tracing
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179952-plasmid-map-sequence-id-354333

    Enlarge

  9. ePop

    Plasmid
    #50953
    Purpose
    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
    Depositor
    Lingchong You
    Article
    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:
    Inserts
    E lysis protein
    LuxI
    LuxR
    Use
    Tags
    Expression
    Mutation
    Early stop codon introduced in mutated luxR resul…
    Promoter
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50953-plasmid-map-sequence-id-454119

    Enlarge

  10. pNLF1W

    Plasmid
    #136404
    Purpose
    Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    136404-plasmid-map-sequence-id-271260

    Enlarge

  13. pSBK.134

    Plasmid
    #187219
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectively
    Depositor
    Seth Shipman
    Article
    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:
    Insert
    A: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    A: pTet*; B: pBetI
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187219-plasmid-map-sequence-id-367486

    Enlarge

  14. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PEM7 and unknown
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

    Enlarge

  15. N-BLa 1.2

    Plasmid
    #88999
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.2 fusion protein
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    88999-plasmid-map-sequence-id-179971

    Enlarge

  18. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

    Enlarge

  19. pRARE-MBP-DEST

    Plasmid
    #84650
    Purpose
    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.
    Depositor
    Neil Olszewski
    Article
    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:
    Type
    Empty backbone
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    84650-plasmid-map-sequence-id-162112

    Enlarge

  24. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

    Enlarge

  27. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

    Enlarge

  28. pECIA14

    Plasmid
    #47051
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Alkaline Phosphatase (human, placental), Flag, HR…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47051-plasmid-map-sequence-id-67169

    Enlarge

  29. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

    Enlarge

  30. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Promoter
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

    Enlarge

  31. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

    Enlarge

  33. AEB-No-aptamer-controlmRFP1

    Plasmid
    #63848
    Purpose
    This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    J23100
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63848-plasmid-map-sequence-id-106571

    Enlarge

  36. pMuLE Lenti Dest Neo

    Plasmid
    #62178
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62178-plasmid-map-sequence-id-101462

    Enlarge

  39. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid
    #172501
    Purpose
    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 full locus with Golden gate acceptor site for spacer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172501-plasmid-map-sequence-id-344918

    Enlarge

  40. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Article
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

    Enlarge

  41. YFVdelCMENS1-Cre

    Plasmid
    #179954
    Purpose
    pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Cre
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    YFV-17D lacking C, M, E and NS1 proteins but with Cre
    Use
    Transneuronal tracing
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179954-plasmid-map-sequence-id-366320

    Enlarge

  42. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Promoter
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

    Enlarge

  44. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

    Enlarge

  46. pCSF107mT-GATEWAY-3'-LAP tag

    Plasmid
    #67618
    Purpose
    A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology
    Depositor
    Todd Stukenberg
    Article
    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi
    Type
    Empty backbone
    Use
    In vitro transcription or translation off of an s…
    Tags
    C-termnal LAP (eGFP-TEV-S)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and SP6
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10101 - 10150 of 10805 results