We narrowed to 13,583 results for: TIM

1. pALPS tat-P2A-rev

   Plasmid

   #101331

   Purpose

   Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 tat and rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT3-Neo

   Plasmid

   #69123

   Purpose

   Sleeping Beauty (SB) transposon with G418 resistance

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Insert

   Neo (codon optimized for human)
   

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ292

   Plasmid

   #129672

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   AaCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-dSa-VPR mini.-syn pA

   Plasmid

   #99689

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLN599 (pFuGW-hUbCp-hSTE1)

   Plasmid

   #105206

   Purpose

   ubiquitin C promoter drives STE expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   hUbCp-hSTE1
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. CH00X

   Plasmid

   #73517

   Purpose

   TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::TagRFP-T::PH::tbb-2
   

   Use

   Tags

   TagRFP-T

   Expression

   Mutation

   Promoter

   mes-5

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pHCMV_SARS-CoV_S

   Plasmid

   #207306

   Purpose

   Mammalian expression of Human SARS coronavirus S protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SARS-CoV_S (S Human SARS coronavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TOPO rtTA3-2A-Cre

    Plasmid

    #68448

    Purpose

    TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    rtTA3-2A-Cre
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in mice

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymVenus-URA3

    Plasmid

    #168058

    Purpose

    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    ymVenus

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PARP15-c020

    Plasmid

    #173101

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N78-S267, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-L1PA8CHmneo

    Plasmid

    #69608

    Purpose

    Expresses a neomycin tagged codon optimized L1PA8

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1PA8
    

    Use

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSZT025

    Plasmid

    #140033

    Purpose

    KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIntegrase_11

    Plasmid

    #60582

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS403red-­luxTA2-­4F

    Plasmid

    #166030

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS405red-­phlTA2-­1E

    Plasmid

    #166029

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Pprm1-luciferase; his-3

    Plasmid

    #137728

    Purpose

    Communication-activated luciferase in Neurospora crassa

    Depositor

    Louise Glass

    Article

    Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

    Inserts

    Luciferase
    Pprm-1 Promoter
    

    Use

    Luciferase; Neurospora crassa expression

    Tags

    Expression

    Mutation

    Codon-optimized for N. crassa

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEG BacMam C term 3C eGFP myc

    Plasmid

    #160685

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    His8 eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRN3P_T3_BE3_IVT

    Plasmid

    #177015

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE3
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-mH6-Cas12i2

    Plasmid

    #120883

    Purpose

    Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIntegrase_4

    Plasmid

    #60576

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGK415-­luxTA2-­4F

    Plasmid

    #165971

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJ204-loxP-100RPU.2-coEPO-lox2272

    Plasmid

    #154828

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    coEPO
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Blat-BSD

    Plasmid

    #163791

    Purpose

    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p33Fn

    Plasmid

    #158155

    Purpose

    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Rice codon optimized

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Integrin alpha5(1-623)-AHCys

    Plasmid

    #27301

    Depositor

    Timothy Springer

    Article

    Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

    Insert

    integrin alpha 5 (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alpha 5(1-623)+GG+TEV+GGEN+AHCys

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pNC1136

    Plasmid

    #41562

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

37. human b1 ecto-pIRES

    Plasmid

    #113387

    Purpose

    Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human beta 1 ectodomain
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSR33

    Plasmid

    #69254

    Purpose

    phsp16.2::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.2

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTH760-CEN-mCherry_v3

    Plasmid

    #40598

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mCherry ORF partially codon optimised for yeast e…

    Promoter

    GPD (TDH3)

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FH95pUp95GW (B4)

    Plasmid

    #74013

    Purpose

    lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

    Depositor

    Oliver Schluter, Robert Malenka, Weifeng Xu

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pGEX-parkin 77-465

    Plasmid

    #45970

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 77-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

    Plasmid

    #45578

    Purpose

    Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLN429 (FuGW-G8p-mKate2)

    Plasmid

    #105185

    Purpose

    Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pfTRAP

    Plasmid

    #50048

    Purpose

    Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    plamodium falciparum TRAP (TRAP Synthetic)
    

    Use

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only