We narrowed to 12,598 results for: 112
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Plasmid#166896Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTagDepositorInsertp58 (Lman1 Rat)
TagsHaloTagExpressionMammalianMutationL405Q, R419S- please see depositor commentsPromoterCMVAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-150_175-470)
Plasmid#170537PurposeExpresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsFLAG tagExpressionMammalianMutationdeleted amino acids 151-175PromoterCAG promoterAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-FAF1-Strep-HA
Plasmid#113486PurposeExpression of human FAF1 with C-terminal strep-HA tagDepositorInsertFAF1 (FAF1 Human)
Tags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK3 K261R
Plasmid#80875Purposemammalian expression of ALK3 K261RDepositorInsertALK3 (BMPR1A Human)
TagsHAExpressionMammalianMutationK261R (Kinase inactive)PromoterCMVAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC6A14_STOP
Plasmid#161031PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC6A14 (SLC6A14 Human)
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)
Plasmid#139447PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
fck-Jaws-GFP-ER2
Plasmid#65013PurposeLentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.DepositorInsertJaws-GFP-ER2
UseLentiviralTagsER2 and GFPExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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dCas9-NLS-gs10-cLYN268
Plasmid#188259PurposePlasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2975
Plasmid#144451PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-APP751
Plasmid#193772PurposeEntry vector for human APP isoform having 751 amino acid residuesDepositorInsertAPP (APP Human)
Available SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF706
Plasmid#88037PurposeDonor Vector containing ZNF706 transcription factor, part of the Human TFome CollectionDepositorInsertZNF706 (ZNF706 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3239
Plasmid#144715PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)
Plasmid#139443PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterSFFVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
fck-Jaws-KGC-GFP-ER2
Plasmid#65012PurposeLentivirus-mediated expression of Jaws under the CaMKII promoter.DepositorInsertJaws-KGC-GFP-ER2
UseLentiviralTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mCherry-p115(S240A)
Plasmid#181997PurposeMammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.DepositorInsertmCherry-p115(S240A) (ARHGEF1 Human)
TagsmCherryExpressionMammalianMutationSerine 240 changed to alanine in p115.PromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.ARID3a.IRES.GFP
Plasmid#169300PurposeConstitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent proteinDepositorAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#174666PurposeDoxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent proteinDepositorAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only