We narrowed to 7,466 results for: addgene/1000

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pMSCV-IRES-GFP-MD2 (C95A)-HA

Plasmid

#133066

Purpose

Retroviral expression of mouse MD2 (C95A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (C95A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C95A

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1809_pBS_cdc42E6-boxB

Plasmid

#118609

Purpose

cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E6 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1807_pBS_cdc42E7-boxB

Plasmid

#118612

Purpose

cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR141

Plasmid

#106045

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR141 (GPR141 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19_T790M_28bp

Plasmid

#114721

Purpose

28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1178

Plasmid

#111104

Purpose

Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1224

Plasmid

#111105

Purpose

Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790M_28bp

Plasmid

#114719

Purpose

4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Thy-C73A-HA

Plasmid

#111542

Purpose

For expression of human KCNB1 in mouse brain

Depositor

Federico Sesti

Article

Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

Insert

thy1.2-C73A-HA (KCNB1 Human)

Use

Mouse Targeting

Tags

HA

Mutation

C73A

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-Stx3S-2xMyc/His

Plasmid

#99744

Purpose

Expresses human Stx3S with a C-term myc-myc-his tag in mammalian cells.

Depositor

Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

Stx3S (STX3 Human)

Tags

myc-myc-his

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FNBP1-1/1

Plasmid

#91552

Purpose

Protein expression and purification of human SH3 domain construct FNBP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FNBP1-1/1 (FNBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB2-2/2

Plasmid

#91507

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-2/2 (CACNB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB2-1/2

Plasmid

#91314

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-1/2 (CACNB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG4-1/1

Plasmid

#91266

Purpose

Protein expression and purification of human SH3 domain construct DLG4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG4-1/1 (DLG4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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YCe2741 HC_Kan_EF1ap_p18

Plasmid

#100698

Purpose

part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EF1a promoter (EEF1A1 Human)

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pPN268

Plasmid

#91631

Purpose

Express sgRNA targeting human KCNB1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KCNB1 (KCNB1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN160

Plasmid

#91579

Purpose

Express sgRNA targeting human CACNB2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CACNB2 (CACNB2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN161

Plasmid

#91580

Purpose

Express sgRNA targeting human CACNB2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CACNB2 (CACNB2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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