We narrowed to 8,036 results for: Dos;

1. pPyCAG-NE-mKate2-IRES-Hph

   Plasmid

   #183616

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

   Insert

   mKate2
   

   Use

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CFP-FRBx5

   Plasmid

   #103774

   Purpose

   expresses CFP-FRBx5 protein in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   CFP-FRBx5
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-ZZ-TEV-SNAPf-BICD2N(1-400)

   Plasmid

   #111857

   Purpose

   N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICD2N(1-400)
   

   Use

   Tags

   His-tag, SNAPf-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   Optimised for Sf9 expression

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPmacroH2A.1-GS3

   Plasmid

   #204759

   Purpose

   Expression of Cas9 and human macroH2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   macroH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA_DNMT3b

   Plasmid

   #41823

   Purpose

   Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_DNMT3b (DNMT3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-GFP-PQRv3-RFP

   Plasmid

   #73951

   Purpose

   Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

   Depositor

   Brian Chen

   Article

   Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

   Inserts

   sfGFP (superfolder GFP)
   RFP
   

   Use

   Tags

   A residual PQR peptide wii be left at the C-termi…

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin and Chicken beta actin (shared…

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pHH0103_YWHAQ_14-3-3

   Plasmid

   #109943

   Purpose

   Protein expression and purification of YWHAQ_14-3-3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   YWHAQ_14-3-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_antiCD19_Z3_Gal4VP64

   Plasmid

   #169916

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMP-SUZ12_1

   Plasmid

   #36390

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. MmSphk2

    Plasmid

    #118598

    Purpose

    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMP-EZH1_2

    Plasmid

    #36360

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pSMP-Dot1L_2

    Plasmid

    #36340

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Dot1L (DOT1L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSMP-EZH1_3

    Plasmid

    #36361

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pHH0103_S100A11_S-100

    Plasmid

    #109804

    Purpose

    Protein expression and purification of S100A11_S-100

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    S100A11_S-100 (S100A11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

    Plasmid

    #78348

    Purpose

    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-KRAB
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_NR3C1_Hormone-recep

    Plasmid

    #110018

    Purpose

    Protein expression and purification of NR3C1_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR3C1_Hormone-recep (NR3C1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSMP-SUZ12_2

    Plasmid

    #36391

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pJR2956

    Plasmid

    #122533

    Purpose

    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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