We narrowed to 7,520 results for: Ski;

1. pS23.U6<ActB.mus_C-term-KI_gRNA

   Plasmid

   #207408

   Purpose

   gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   ActB
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1 FRB-myc-RagC

   Plasmid

   #112741

   Purpose

   express FRB-myc-RagC

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   FRB-myc-RagC (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   FRB, myc

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Nck-SH2

   Plasmid

   #46457

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Nck (NCK1 Human)
   

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 275-377

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX ShcA-SH2

   Plasmid

   #46486

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   ShcA (SHC1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 476-584

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO_CEP41_HaloTag9

   Plasmid

   #169331

   Purpose

   Expression of HaloTag9 located at the microtubules of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag9 (CEP41 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-sgRNA_Ago2_3

   Plasmid

   #73531

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HSF1 KO

   Plasmid

   #200207

   Purpose

   gRNA for HSF1 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF1 KO (Hsf1 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS NL4-3 envFS eGFP 5'CMV Δtat

   Plasmid

   #101347

   Purpose

   tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta Tat

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-sgRNA_Ago2_4

   Plasmid

   #73532

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPHR-EYFP-NLS

    Plasmid

    #103820

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-NLS (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe_puro_DEST_Flag_PRKCI

    Plasmid

    #45263

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PKCI (PRKCI Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pEY61

    Plasmid

    #191058

    Purpose

    acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(3.7k) (acr-2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ama1CD4d3+4-blac

    Plasmid

    #36146

    Purpose

    EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-blac

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ama1 (PF3D7_1133400 Plasmodium falciparum)
    

    Tags

    beta-lactamase, pentamerization domain of rat car…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX SH2A-SH2

    Plasmid

    #46481

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2A (SH2D4A Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 338-445

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Slap2-SH2

    Plasmid

    #46501

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Slap2 (SLA2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 84-197

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Lnk-SH2

    Plasmid

    #46451

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Lnk (Sh2b3 Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 325-466

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNAS1b split mCherry NEDD4-2 WW2

    Plasmid

    #111883

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Tags

    Split mCherry system: NEDD4-2 WW2 fused to C-term…

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIN40C.SFFV.mTagBFP2.let-7c

    Plasmid

    #169310

    Purpose

    Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    let-7c (MIRLET7C Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SIN40C.SFFV.dTomato.miR-125b-2

    Plasmid

    #169302

    Purpose

    Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-125b-2 (MIR125B2 Human)
    

    Use

    Lentiviral

    Tags

    dTomato

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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