We narrowed to 5,622 results for: crispr cas9 grna plasmid

1. 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

   Plasmid

   #195495

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 53BP1 N-terminal sgRNA

   Plasmid

   #207091

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGGGAGCAGATGGACCCTAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATM N-terminal sgRNA

   Plasmid

   #207089

   Purpose

   pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ATCATTAAGTACTAGACTCA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. RNF168 C-terminal sgRNA

   Plasmid

   #207100

   Purpose

   pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GAGATGCACAAAGTAAGGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. RIF C-terminal sgRNA

   Plasmid

   #207096

   Purpose

   pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AATACTAAATAGAATTTTCA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. REV7 C-terminal sgRNA

   Plasmid

   #207095

   Purpose

   pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GCTCATAAAGGCAGCTGAGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SHLD3 N-terminal sgRNA

   Plasmid

   #207094

   Purpose

   pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TTACTGCAGAATGACTACAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. NBS1 C-terminal sgRNA

   Plasmid

   #207092

   Purpose

   pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TAAAAAGGAGAAGATAACTG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. RNF169 C-terminal sgRNA

    Plasmid

    #207099

    Purpose

    pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ACACTTCATTAGGTGCTACT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SHLD1 N-terminal sgRNA

    Plasmid

    #207101

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATGGCAGGACTATGGCAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATM C-terminal sgRNA

    Plasmid

    #207097

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTCTAAAGGCTGAATGAAA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SHLD2 N-terminal sgRNA

    Plasmid

    #207098

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTTTATCAGAAATCATGAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MDC1 N-terminal sgRNA

    Plasmid

    #207090

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTATCCTTCCCAGATCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEVA47_dCas9-GFP_AAN_4

    Plasmid

    #231415

    Purpose

    This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    dCas9
    GFP
    sgRNA-X
    sgRNA-Y
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2ss-U6-sgHTT1-7sk-sgCas9

    Plasmid

    #190900

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (HTT Human, spCas9)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and 7sk

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVss-U6-sgHTT51-7sk-Cas9

    Plasmid

    #190901

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (Htt Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and 7sk

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNOC-CRISPR-GFP

    Plasmid

    #100009

    Purpose

    Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-GFP

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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