164,313 results

1. FH-TET3-pEF

   Plasmid

   #49446

   Purpose

   This plasmid expresses full-length human TET3.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   Tet methylcytosine dioxygenase 3 (TET3 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. alpha7nACHR_Nacho_GCAMP7s

   Plasmid

   #182940

   Purpose

   alpha7 nACHR expression in mammalian cell lines with calcium reporter

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-MCS2

   Plasmid

   #46954

   Purpose

   Cloning plasmid for the generation of Knock-In in human cells using rAAV.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR26 CAG/GFP Asc

   Plasmid

   #74285

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MCS-BioID2-HA

   Plasmid

   #74224

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. OpenCRISPR-1 sgRNA-12nt stem

   Plasmid

   #221567

   Purpose

   Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 12nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. GFP-C1-PLCdelta-PH

   Plasmid

   #21179

   Purpose

   encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

   Depositor

   Tobias Meyer

   Article

   Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

   Insert

   PLCdelta (PLCD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of human PLC delta, amino acids Met1–Il…

   Promoter

   Available Since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. hNav1.8_pcDNA3.1

   Plasmid

   #182847

   Purpose

   Expresses hNav1.8 in mammalian cells

   Depositor

   Paul DeCaen

   Article

   McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

   Insert

   SCN10A (SCN10A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJFRC19-13XLexAop2-IVS-myr::GFP

    Plasmid

    #26224

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    myr::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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