We narrowed to 7,223 results for: ALP

1. myr-FLAG-AKT1-pcw107

   Plasmid

   #64606

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and myristoylated

   Expression

   Mutation

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pHAGE-PIK3CA-E542K

   Plasmid

   #116479

   Purpose

   Lentiviral expression of PIK3CA E542K

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E542K

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_PIK3CA_p.H1047R

   Plasmid

   #82824

   Purpose

   Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   H1047R

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-2dV-Camui (T305D/T306D)

   Plasmid

   #220368

   Purpose

   T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Use

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed both Threonine 305 and 306 to aspartic ac…

   Promoter

   CAG

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_PAK1_WT

   Plasmid

   #82220

   Purpose

   Gateway Donor vector containing PAK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PAK1 (PAK1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-PIK3CA-Q546R

   Plasmid

   #116530

   Purpose

   Lentiviral expression of PIK3CA Q546R

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Q546R

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.iGluSnFR3.v857.SGZ

   Plasmid

   #178334

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_PIK3CA_WT

   Plasmid

   #81736

   Purpose

   Gateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIGF1_P1 Promoter-FLuc

   Plasmid

   #154266

   Purpose

   Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   IGF-1 canonical P1 Promoter (IGF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human IGF-1 P1 Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-PIK3CA-Q546K

    Plasmid

    #116527

    Purpose

    Lentiviral expression of PIK3CA Q546K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Q546K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.GFAP.iGluSnFR3.v857.SGZ

    Plasmid

    #178337

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe-Neo-Myr-Flag-AKT1

    Plasmid

    #15266

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pB-Tet-On-HTR6-RhoA sensor

    Plasmid

    #189614

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6 (HTR6 Human)
    

    Use

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Tet3G

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-PIK4CA

    Plasmid

    #23541

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK4CA (PI4KA Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pGL3-mFgf21pro2-luc

    Plasmid

    #101798

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid

    #85756

    Purpose

    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Inserts

    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    

    Use

    CRISPR

    Tags

    Csy4, Csy4 recognition sequence, FokI fusion via …

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CMV and U6

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-mFgf21pro3-luc

    Plasmid

    #101799

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV6-MPP8-10xHis

    Plasmid

    #194178

    Purpose

    Expresses MPP8-10xHis tagged

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL-CRISPR.EFS.PAC

    Plasmid

    #57828

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-SpCas9D10A nickase

    Plasmid

    #216737

    Purpose

    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMVd1

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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