We narrowed to 52,924 results for: plasmid
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Plasmid#86778PurposeExpresses Francisella tularensis Histidine Acid Phosphatase under T7 with HIS tagDepositorInsertHistidine Acid Phosphatase
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pGBR22-pGEM-T
Plasmid#231055PurposeExpresses Montipora efflorescens GFP-like chromoprotein under T7 with a 6xHIS tagDepositorInsertGFP-like chromoprotein
UseCloningTags6x HISExpressionMutationPromoterT7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG
Plasmid#229223PurposeFor expression of T. elongatus KaiB protein mutantDepositorInsertkaiB (kaiB T. elongatus)
UseTagsFLAG and SUMO-FLAGExpressionBacterialMutation1-94 (truncated), Y8A, Y94APromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-GFP_CBLN3
Plasmid#227950PurposeCo-localisation experimentDepositorInsertCBLN3 (CBLN3 Human)
UseLentiviralTags3xFLAG-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpec5'
Plasmid#228549PurposeTemplate for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpec3'
Plasmid#228548PurposeTemplate for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmidDepositorInsertTruncated 3' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3566_GST-TEV-Spt6p-tSH2-1223-1451
Plasmid#227085PurposeBacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10
Plasmid#219699PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tagDepositorInsertpSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pIND4-GFP-MmXYRS
Plasmid#222745PurposeExpresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinkerDepositorInsertssfGFP
MmXYRS
Mm Pyl tRNA
UseTags6xHisExpressionMutationContains the following substitutions: V346A, W348…PromoterrrnBAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-35aaNSP1noMSA11-NSP5(io)
Plasmid#220827PurposeExpress the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length recoded NSP5 proteinDepositorInsertRotavirus SA11 35aaNSP1noM-NSP5(io)
UseOtherTagsExpressionMutationthe 35aa NSP1 lacks all MethioninesPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-35aaNSP1noMSA11-NSP5(wt)
Plasmid#220826PurposeExpress the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length SA11 NSP5 proteinDepositorInsertRotavirus SA11 35aaNSP1noM-NSP5(wt)
UseOtherTagsExpressionMutationthe 35aa NSP1 lacks all MethioninesPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)-FLAG
Plasmid#217329PurposePurification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXR004_puroR
Plasmid#219820PurposehU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)
Plasmid#219689PurposeLevel 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB (mtagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lmod1-BirA
Plasmid#221509PurposeFlag-tagged biotinylation enzyme for co-expression of Lmod1DepositorInsertLmod1 (Lmod1 Mouse)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1_BirA_Nterm
Plasmid#221523PurposeFlag-tagged biotinylation enzyme for co-expression of Synj1DepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1_BirA_Cterm
Plasmid#221522PurposeFlag-tagged biotinylation enzyme for co-expression of Synj1DepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
Plasmid#217970PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.DepositorInsert2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4
Plasmid#219711PurposeLevel 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-eGFP-oPRE
Plasmid#218681PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-mEGFP-P2A-otch25hb4
Plasmid#217484PurposeExpression of Green Fluorescent Protein - P2A - full length cholesterol 25-hydroxylase-like protein from chinook salmonDepositorInsertmEGFP-P2A-otch25hb4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
UseTagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST-IRES-iRFP670
Plasmid#216344PurposeFor testing localization of pFAST and iRFP670 proteins to endoplasmic reticulumDepositorInsertPRL_pFAST-IRES-CANX_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmVen-C17orf53
Plasmid#211419Purposemammalian expression of human C17orf53 N-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-C17orf53
Plasmid#211418Purposemammalian expression of human C17orf53 N-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C16orf59-mCer
Plasmid#211424Purposemammalian expression of human C16orf59 C-terminally fused to mCeruleanDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53
Plasmid#211445Purposegeneration of Bacmid for insect cell expression of C17orf53DepositorInsertC17orf53 (HROB Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C16orf59-mVen
Plasmid#211425Purposemammalian expression of human C16orf59 C-terminally fused to mVenusDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR3cDual-Wnk
Plasmid#203976PurposeEnrty clone for Gateway cloningDepositorInsertWnk (Wnk Fly)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-ELMOD2
Plasmid#201896PurposeUsed for expression of human protein ELMOD2 in mammalian cellsDepositorInsertELMOD2 (Elmod2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-ELMOD1
Plasmid#201894PurposeUsed for expression of human protein ELMOD1 in mammalian cellsDepositorInsertELMOD1 (ELMOD1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15(CO)
Plasmid#201879PurposeUsed for bacterial expression of human protein ARL15DepositorInsertARL15 (ARL15 Human)
UseTagsExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4-ELMOD3
Plasmid#201893PurposeUsed for bacterial expression of human protein ELMOD3DepositorInsertELMOD3 (ELMOD3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL14-His6
Plasmid#201878PurposeUsed for bacterial expression of human protein ARL14DepositorInsertARL14 (ARL14 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL8A-His6
Plasmid#201872PurposeUsed for bacterial expression of human protein ARL8ADepositorInsertARL8A (ARL8A Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL3-His6
Plasmid#201868PurposeUsed for bacterial expression of human protein ARL3DepositorInsertARL3 (ARL3 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL9-His6
Plasmid#201874PurposeUsed for bacterial expression of human protein ARL9DepositorInsertARL9 (ARL9 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ctag
Plasmid#195397PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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NXPH1_22-271_AviN_HisC
Plasmid#195872PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNXPH1 (NXPH1 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
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sfCherry11-Ex1-17-PTPN22
Plasmid#195387PurposeMammalian expression of a human PTPN22 mutant (exon 1-17) with sfCherry(11) tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagssfCherry(11)ExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17PromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-DmNot11_1-214_AF
Plasmid#148648PurposeBacterial Expression of HsNot11_1-214DepositorInsertHsNot11_1-214 (CNOT11 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-DmNot11_16-214_AF
Plasmid#148649PurposeBacterial Expression of HsNot11_16-214DepositorInsertHsNot11_16-214 (CNOT11 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsNot11_257-320_AF
Plasmid#148650PurposeBacterial Expression of HsNot11_257-320DepositorInsertHsNot11_257-320 (CNOT11 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only