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YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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YSBE 557 : pNAT_GFP

Plasmid

#92085

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET-hisT4DNALig

Plasmid

#216671

Purpose

Expresses his-tagged T4 DNA ligase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 DNA Ligase (30 T4 phage)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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LRT_RTctrl

Plasmid

#214074

Purpose

Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LRT

Plasmid

#214071

Purpose

Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-enAsCas12f

Plasmid

#204637

Purpose

Expression of enAsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

enAsCas12f

Tags

NLS

Expression

Mammalian

Mutation

D196K, N199K, G276R, N328G, D364R

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pC007 - pRFP constitutive expression

Plasmid

#79156

Purpose

RFP constitutively expressed

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

RFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLN-AIDA-Tyr1

Plasmid

#192831

Purpose

IPTG inducible AIDA-Tyrosinase expression

Depositor

André Studart

Article

Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

Insert

AIDA-Tyr1

Expression

Bacterial

Promoter

pL-lac

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-TEAD4

Plasmid

#24638

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD-4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1beta AS4(-)

Plasmid

#59409

Purpose

Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Nrxn1beta AS4(-) (Nrxn1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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ARTi

Plasmid

#214064

Purpose

Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTW199b2

Plasmid

#136928

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_GFP

Plasmid

#127108

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

GFP

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE hBRM

Plasmid

#1961

Depositor

Robert Kingston

Article

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRM (SMARCA2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Tags

FIREmate

Expression

Mammalian

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1160 mCherry-FIREtag

Plasmid

#205164

Purpose

Expresses mCherry -FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBT120a17

Plasmid

#138510

Purpose

P1

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

gIII-xLuxAB

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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Human Bcl-2/pcDNA3

Plasmid

#19279

Depositor

Philip Leder

Article

Fantin et al Cancer Res. 2004 Jan 1. 64(1):329-36.

Insert

human Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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