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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,707 results for: SHE

Showing: 6151 - 6180 of 17707 results

  1. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid
    #185060
    Purpose
    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185060-plasmid-map-sequence-id-375730

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  2. pcDNA3.1_RapACR_T111C_N248Q-EYFP

    Plasmid
    #119197
    Purpose
    Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q
    Depositor
    John Spudich
    Insert
    Anion channelrhodopsin RapACR fast mutant T111C_N248Q
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    changed threonine 111 to cysteine and asparagine …
    Promoter
    CMV
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    119197-plasmid-map-sequence-id-230387

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  3. PTPN7

    Plasmid
    #38945
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN7 (PTPN7 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pT7-7 asyn S87E/S129E

    Plasmid
    #36064
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87E/S129E (SNCA Human)
    Expression
    Bacterial
    Mutation
    S87E/S129E
    Promoter
    T7
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36064-plasmid-map-sequence-id-44521

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  6. PHGDH

    Plasmid
    #38841
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHGDH (PHGDH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pGL3-IL-4

    Plasmid
    #40570
    Depositor
    Mark Kaplan, Alexander Dent
    Insert
    IL-4 promoter (Il4 Mouse)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    contains 250 bp of the proximal mouse IL-4 promot…
    Promoter
    mouse IL-4
    Available Since
    Oct. 12, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. TLCV2 sgAAVS1

    Plasmid
    #127099
    Purpose
    Encodes gRNA for human AAVS1.
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    Use
    Lentiviral
    Promoter
    U6
    Available Since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127099-plasmid-map-sequence-id-246497

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  9. hMYF5-3xHA-IRES-hrGFP (#397)

    Plasmid
    #78331
    Purpose
    Overexpression of human MYF5 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1_hSMAD4

    Plasmid
    #212710
    Purpose
    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens SMAD4 (SMAD4 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212710-plasmid-map-sequence-id-448252

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  12. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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  13. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid
    #78332
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Promoter
    TRE2
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Expression
    Bacterial
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available Since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  18. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid
    #44089
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44089-plasmid-map-sequence-id-61674

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  19. MAGEA4

    Plasmid
    #39001
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA4 (MAGEA4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39001-plasmid-map-sequence-id-218943

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  20. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dTET1
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104413-plasmid-map-sequence-id-204141

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  21. pBABEpuro-CRAF_S259A

    Plasmid
    #51127
    Purpose
    mammalian expression of wt CRAF S259A
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    S259A
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51127-plasmid-map-sequence-id-77385

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  22. PTPRN2

    Plasmid
    #38891
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRN2 (PTPRN2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pFR022

    Plasmid
    #134228
    Purpose
    bacterial expression of KNL1
    Depositor
    Iain Cheeseman
    Insert
    hKNL1 aa 2106-2316 (KNL1 Human)
    Expression
    Bacterial
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    134228-plasmid-map-sequence-id-271855

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  24. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. PRDM11

    Plasmid
    #32858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM11 (PRDM11 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 79-314
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. RPS6KA1

    Plasmid
    #39021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RPS6KA1 (RPS6KA1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. MIGR1-Meis1a

    Plasmid
    #78148
    Purpose
    retroviral expression of Meis1a and GFP
    Depositor
    Francesco Blasi
    Insert
    Meis1a (Meis1 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Available Since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid
    #185699
    Purpose
    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter
    Depositor
    Lynette Lim
    Inserts
    Tdtomato,
    Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP and T2A
    Expression
    Mammalian
    Promoter
    mDlx
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185699-plasmid-map-sequence-id-366333

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  30. SETD3

    Plasmid
    #32873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD3 (SETD3 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6151 - 6180 of 17707 results