161,873 results

1. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 and H1

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCpf1b-sp

   Plasmid

   #122188

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTW2066

   Plasmid

   #236575

   Purpose

   ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

   Plasmid

   #175525

   Purpose

   Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SARS-Spike

   Plasmid

   #145031

   Purpose

   Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV Spike
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGEX-2T PTP-1B

   Plasmid

   #8602

   Depositor

   Ben Neel

   Insert

   PTP-1B (PTPN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB411

   Plasmid

   #74805

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRC-APEX2

   Plasmid

   #72558

   Purpose

   Expression of cytosolic APEX2 in bacteria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A134P relative to APEX

   Promoter

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRFP-Lact-C2

   Plasmid

   #74061

   Purpose

   Fluorescent biosensor for phosphatidylserine.

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 (MFGE8 Bovine)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-Con/Fon-GCaMP6M

    Plasmid

    #137119

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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