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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,707 results for: SHE

Showing: 6181 - 6210 of 17707 results

  1. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Promoter
    GAL1 promoter
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pSBtet-Bla/PAG1-GFP

    Plasmid
    #198220
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198220-plasmid-map-sequence-id-389402

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  3. HaloTag-G protein gamma-7

    Plasmid
    #67040
    Purpose
    G protein gamma-7 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-7 (GNG7 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67040-plasmid-map-sequence-id-115582

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  4. pCB32

    Plasmid
    #111911
    Purpose
    Expresses gRNA to target Nte1 locus and has HygR marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting Nte1 locus
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    SNR52p
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111911-plasmid-map-sequence-id-218313

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  5. pKG210

    Plasmid
    #69761
    Purpose
    Retroviral expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. MLYCD

    Plasmid
    #39081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MLYCD (MLYCD Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation E19A, K20A, E2…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Available Since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

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  8. pLP-mOlig2-ires-gfp

    Plasmid
    #49399
    Purpose
    mammalian bicistronic expression of mouse Olig2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Olig2 (Olig2 Mouse)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP and IRES
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    49399-plasmid-map-sequence-id-316898

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  9. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

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  10. pBABEpuro-CRAF_S257L

    Plasmid
    #51125
    Purpose
    mammalian expression of wt CRAF S257L
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    S257L
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51125-plasmid-map-sequence-id-77383

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  12. pAAV asyn S129E

    Plasmid
    #36068
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129E (SNCA Human)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S129E
    Promoter
    CMV
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    dJMJD2A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104418-plasmid-map-sequence-id-204359

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  14. pZC11 (pFA6a-TEV-6xGly-V5-KanMX)

    Plasmid
    #44097
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    44097-plasmid-map-sequence-id-61694

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  15. PA-TagRFP-Alpha-5-Integrin-12

    Plasmid
    #62791
    Purpose
    Localization: Focal Adhesions, Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Tags
    TagRFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    Ef1-alpha
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  17. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180151
    Purpose
    td-sfGFP cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180151-plasmid-map-sequence-id-355316

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  18. PBC002-A04

    Plasmid
    #58761
    Purpose
    bacterial expression of PDB 4PWY
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PWY (PEX6 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pCB100

    Plasmid
    #69746
    Purpose
    Retroviral expression of Centrin 3
    Depositor
    Iain Cheeseman
    Insert
    Centrin 3 (CETN3 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. ADH4

    Plasmid
    #38910
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADH4 (ADH4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. FN 227

    Plasmid
    #50499
    Depositor
    Kenneth Yamada
    Insert
    FN1 fibronectin 1 (FN1 Human)
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    repeats 6-10; D1526E and R1524K
    Promoter
    T7
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. SPSB1

    Plasmid
    #38981
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPSB1 (SPSB1 Human)
    Tags
    6xHis-Tev
    Expression
    Bacterial
    Mutation
    Residues 24-233 of SPSB1 are present in this plas…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38981-plasmid-map-sequence-id-199971

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  23. UXS1

    Plasmid
    #39162
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UXS1 (UXS1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

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  26. pFLAG-6a-Ataxin3Q22-C14A

    Plasmid
    #22127
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    C14A
    Available Since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  27. mEmerald-Enconsin-N-18

    Plasmid
    #62754
    Purpose
    Localization: Microtubule, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    Enconsin (MAP7 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62754-plasmid-map-sequence-id-230170

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  28. PHIP

    Plasmid
    #39049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIP (PHIP Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. TPK1

    Plasmid
    #32865
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TPK1 (TPK1 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pDK714

    Plasmid
    #134230
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK365 tdTomato (short SKAP) (KNSTRN Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Available Since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134230-plasmid-map-sequence-id-272270

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Showing: 6181 - 6210 of 17707 results