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pLenti_CamKIIa_mKate2

Plasmid

#96942

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

far-red fluorescent protein

Use

Lentiviral

Mutation

E87D (please see depositor comments below)

Promoter

CaMKIIa

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Tags

octahistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox1

Plasmid

#213725

Purpose

Mammalian cell expression vector for overexpression of Rbfox1 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox1 (Rbfox1 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox3

Plasmid

#213727

Purpose

Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox3 (Rbfox3 Mouse)

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q

Plasmid

#132545

Purpose

Expresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHis-hMTMR1

Plasmid

#100723

Purpose

Expression of human MTMR1 PH-PTP domain

Depositor

Byung Il Lee

Article

Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

Insert

MTMR1 (MTMR1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 95-607

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pZDonor-Nm-Cas9-gRNA-hU6

Plasmid

#61366

Purpose

empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

Plasmid

#134332

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Lmo

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_TXN_Thioredoxin

Plasmid

#109778

Purpose

Protein expression and purification of TXN_Thioredoxin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TXN_Thioredoxin (TXN Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

Plasmid

#134340

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC1

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-N-CretrcintG

Plasmid

#69572

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - N-CretrcintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-C-CreintG

Plasmid

#69573

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - C-CreintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD_W42A-IFP2.0C

Plasmid

#215751

Purpose

Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD_W42A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139D-GS3

Plasmid

#204748

Purpose

Expression of Cas9 and human H2AX with the S139D mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139D (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139D mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet3 CD Mut

Plasmid

#72222

Purpose

Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET3 catalytic domain (cata…

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pCAGGS-3xFLAG-Rbfox2

Plasmid

#213726

Purpose

Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox2 (Rbfox2 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr3

Plasmid

#108102

Purpose

Retroviral vector for expression of mouse Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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