We narrowed to 5,494 results for: SPI

1. L2374-FRB-GPA-20-11S

   Plasmid

   #222895

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc11S
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L2373-FRB-GPA-20-114

   Plasmid

   #222894

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L2179-FRB-GPA-6-GFPS

   Plasmid

   #222893

   Purpose

   Biosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-6GS-GFPS
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L2092-GPA-FullBRET-Retrovirus-TD142

   Plasmid

   #222877

   Purpose

   Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-NanoLuc-CyOFP1
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXS-IRES-Blast coUCK2 D62A

   Plasmid

   #211530

   Purpose

   Expresses codon optimized catalytic mutant UCK2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UCK2 D62A (UCK2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D62A (catalytic mutant)

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L2110-FKBP-GPA-20-114

   Plasmid

   #222881

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc114
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L2125-FRB-GPA-NLuc-CyOFP1

   Plasmid

   #222885

   Purpose

   Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L2108-FRB-GPA-20-114

   Plasmid

   #222879

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. L2109-FRB-GPA-20-11S

   Plasmid

   #222880

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc11S
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBiFC-VN155(I152L)-SRCR5

    Plasmid

    #207993

    Purpose

    BiFC assay

    Depositor

    Young Tang

    Article

    Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

    Insert

    SRCR5 (CD163 pig)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT-TO-nsp7

    Plasmid

    #201025

    Purpose

    Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet-ON

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ230-D156R

    Plasmid

    #188542

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r entry c…

    Tags

    NLS

    Expression

    Plant

    Mutation

    D156R

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DsRed -7e

    Plasmid

    #191765

    Purpose

    Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Use

    Tags

    N terminal fusion of negatively charged polypetod…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

    Plasmid

    #191481

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR ΔSL1
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA 3.1 BS (-) N protein

    Plasmid

    #188448

    Purpose

    SARS CoV-2 N protein expression

    Depositor

    Ajit Chande

    Article

    Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

    Insert

    SARS-CoV-2 N protein
    

    Use

    Tags

    Strep-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST_CAHS1_PARRC_150-189 (pBS0658)

    Plasmid

    #185201

    Purpose

    For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS1_PARRC_150-189
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ptia1l-hsp-GFP

    Plasmid

    #177837

    Purpose

    Donor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9

    Depositor

    David Kingsley

    Article

    Wucherpfennig et al Nat Ecol Evol. 2022 Sep 1. pii: 10.1038/

    Insert

    tia1l-sgRNA target site-hsp70-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    hsp70

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST_APOE_HUMAN_A234G (pBS0816)

    Plasmid

    #185267

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_A234G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A234G

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

    Plasmid

    #185207

    Purpose

    For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    D4NWF2_DrHD_b03_dom3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST_AfLEA1.1 (pBS0741)

    Plasmid

    #185233

    Purpose

    For the mammalian expression of the brine shrimp protein AfLEA1.1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfLEA1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge