159,557 results

1. 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

   Plasmid

   #89060

   Purpose

   AAV-gRNA cloning vector with GFP reporter

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY0551-Luciferase RADARS

   Plasmid

   #182538

   Purpose

   Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Insert

   RADARS Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST39-pNL29

   Plasmid

   #91696

   Purpose

   Expresses Mega GVs in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

   Insert

   Mega GV gene cluster (from pNL29) in pST39 backbone
   

   Use

   Tags

   No tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYTK002

   Plasmid

   #65109

   Purpose

   Encodes ConLS as a Type 1 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   ConLS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CA-RIT-NFAT1 (IRES-GFP)

   Plasmid

   #85181

   Purpose

   Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

   Depositor

   Anjana Rao

   Article

   Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

   Insert

   CA-RIT-NFAT1 (Nfatc2 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

   Promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pBT272_(pRosa26-GTET)

   Plasmid

   #36882

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   tdT-3Myc
   insulator
   diphteria toxin A
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES2-eGFP-Ofd1-Myc

   Plasmid

   #24560

   Depositor

   Jeremy Reiter

   Article

   Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-Nrxn1beta AS4(-)

   Plasmid

   #59409

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCardinal-Lifeact-7

   Plasmid

   #54663

   Purpose

   Localization: Actin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mCardinal-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pmScarlet-i_alphaTubulin_C1

    Plasmid

    #85047

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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