We narrowed to 15,738 results for: LEA

1. pNW3

   Plasmid

   #53372

   Purpose

   Csy4 and Cas9 D10A nickase expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9n (D10A)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMJ823

   Plasmid

   #39313

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (lin2744 Listeria innocua)
   

   Use

   CRISPR

   Tags

   MBP and thioredoxin-His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

3. MSCV miRNA control plasmid

   Plasmid

   #31508

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. Caspase-6

   Plasmid

   #100102

   Purpose

   Bacterial expression of Caspase-6

   Depositor

   Jim Wells

   Article

   Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

   Insert

   Caspase-6 (CASP6 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pExpreS2-1

   Plasmid

   #175360

   Purpose

   ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOCC153

   Plasmid

   #118901

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2T-CMV-eA3AmaxNG-BlastR

   Plasmid

   #152999

   Purpose

   C-to-T base editor, NG PAM

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-nSpCas9 NG-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRG645_lexO-AscI_LexA-TF_LEU2MX

   Plasmid

   #154813

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ-NF-L3-NF-L1-dMb2Cas12a

    Plasmid

    #177686

    Purpose

    Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    2xFokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSGKP_AcsgRNA

    Plasmid

    #203807

    Purpose

    pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcgRNA
    

    Use

    CRISPR

    Promoter

    J23119

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 FLIPE-1m

    Plasmid

    #13544

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-ninein coiled-coil 2

    Plasmid

    #73522

    Purpose

    Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

    Depositor

    Michel Bornens

    Article

    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

    Insert

    ninein coiled-coil 2 (Nin Mouse)
    

    Tags

    EGFP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-ninein coiled-coil 1

    Plasmid

    #73521

    Purpose

    Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

    Depositor

    Michel Bornens

    Article

    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

    Insert

    ninein coiled-coil 1 (Nin Mouse)
    

    Tags

    EGFP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

15. YTIP-GFP

    Plasmid

    #42238

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YTIP-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2T-CMV-eA3Amax-T31A-NG-BlastR

    Plasmid

    #153000

    Purpose

    C-to-T/G/A base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T31A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpRY

    Plasmid

    #198544

    Purpose

    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-ERK2 T183AY185F

    Plasmid

    #118299

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 T183AY185F (Mapk1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T183AY185F

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xEGFP-mMeikin

    Plasmid

    #174708

    Purpose

    Mammalian expression of mouse Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Tags

    3xEGFP

    Expression

    Mammalian

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSC236

    Plasmid

    #216368

    Purpose

    xhNup155-Nb2i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb2i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge